article · 02/01/2021
Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
Voir chez l’éditeurArticle en PDF
Résumé
Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after ~30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources-particularly oxygen-and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.
Citer cet article
Monmeyran, A., Benyoussef, W., Thomen, P., Dahmane, N., Baliarda, A., Jules, M., Aymerich, S., & Henry, N. (2021). Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel. NPJ Biofilms Microbiomes, 7(1), 64. https://doi.org/10.1038/s41522-021-00233-4@article{Monmeyran2021_372,
author = {Monmeyran, Amaury and Benyoussef, Wafa and Thomen, Philippe and Dahmane, Narimane and Baliarda, Aurélie and Jules, Matthieu and Aymerich, S and Henry, Nelly},
year = {2021},
month = {1},
title = {Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel},
journal = {NPJ Biofilms Microbiomes},
volume = {7},
number = {1},
pages = {64},
abstract = {Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after \textasciitilde{}30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources-particularly oxygen-and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/34354076},
doi = {10.1038/s41522-021-00233-4},
issn = {2055-5008},
}TY - JOUR
AU - Monmeyran, Amaury
AU - Benyoussef, Wafa
AU - Thomen, Philippe
AU - Dahmane, Narimane
AU - Baliarda, Aurélie
AU - Jules, Matthieu
AU - Aymerich, S
AU - Henry, Nelly
PY - 2021
DA - 2021/01/02
TI - Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
JO - NPJ Biofilms Microbiomes
VL - 7
IS - 1
SN - 2055-5008
AB - Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after ~30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources-particularly oxygen-and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.
SP - 64
DO - 10.1038/s41522-021-00233-4
UR - https://www.ncbi.nlm.nih.gov/pubmed/34354076
ER -