article · 01/07/2009
Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder
Résumé
To understand non-trivial biological functions, it is crucial to develop minimal synthetic models that capture their basic features. Here, we demonstrate a sequence-independent, reversible control of transcription and gene expression using a photosensitive nucleic acid binder (pNAB). By introducing a pNAB whose affinity for nucleic acids is tuned by light, in vitro RNA production, EGFP translation, and GFP expression (a set of reactions including both transcription and translation) were successfully inhibited in the dark and recovered after a short illumination at 365 nm. Our results indicate that the accessibility of the protein machinery to one or several nucleic acid binding sites can be efficiently regulated by changing the conformational/condensation state of the nucleic acid (DNA conformation or mRNA aggregation), thus regulating gene activity in an efficient, reversible, and sequence-independent manner. The possibility offered by our approach to use light to trigger various gene expression systems in a system-independent way opens interesting perspectives to study gene expression dynamics as well as to develop photocontrolled biotechnological procedures.
Citer cet article
Estevez-Torres, A., Crozatier, C., Diguet, A., Hara, T., Saito, H., Yoshikawa, K., & Baigl, D. (2009). Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder. Proc. Natl. Acad. Sci. U. S. A., 106(30). https://doi.org/10.1073/pnas.0904382106@article{EstevezTorres2009_95,
author = {Estevez-Torres, Andre and Crozatier, Cecile and Diguet, Antoine and Hara, Tomoaki and Saito, Hirohide and Yoshikawa, Kenichi and Baigl, Damien},
year = {2009},
month = {7},
title = {Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder},
journal = {Proc. Natl. Acad. Sci. U. S. A.},
publisher = {NATL ACAD SCIENCES},
volume = {106},
number = {30},
address = {2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA},
abstract = {To understand non-trivial biological functions, it is crucial to develop minimal synthetic models that capture their basic features. Here, we demonstrate a sequence-independent, reversible control of transcription and gene expression using a photosensitive nucleic acid binder (pNAB). By introducing a pNAB whose affinity for nucleic acids is tuned by light, in vitro RNA production, EGFP translation, and GFP expression (a set of reactions including both transcription and translation) were successfully inhibited in the dark and recovered after a short illumination at 365 nm. Our results indicate that the accessibility of the protein machinery to one or several nucleic acid binding sites can be efficiently regulated by changing the conformational/condensation state of the nucleic acid (DNA conformation or mRNA aggregation), thus regulating gene activity in an efficient, reversible, and sequence-independent manner. The possibility offered by our approach to use light to trigger various gene expression systems in a system-independent way opens interesting perspectives to study gene expression dynamics as well as to develop photocontrolled biotechnological procedures.},
url = {http://www.dx.doi.org/10.1073/pnas.0904382106},
doi = {10.1073/pnas.0904382106},
issn = {0027-8424},
}TY - JOUR
AU - Estevez-Torres, Andre
AU - Crozatier, Cecile
AU - Diguet, Antoine
AU - Hara, Tomoaki
AU - Saito, Hirohide
AU - Yoshikawa, Kenichi
AU - Baigl, Damien
PY - 2009
DA - 2009/07/01
TI - Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder
JO - Proc. Natl. Acad. Sci. U. S. A.
VL - 106
IS - 30
PB - NATL ACAD SCIENCES
SN - 0027-8424
AB - To understand non-trivial biological functions, it is crucial to develop minimal synthetic models that capture their basic features. Here, we demonstrate a sequence-independent, reversible control of transcription and gene expression using a photosensitive nucleic acid binder (pNAB). By introducing a pNAB whose affinity for nucleic acids is tuned by light, in vitro RNA production, EGFP translation, and GFP expression (a set of reactions including both transcription and translation) were successfully inhibited in the dark and recovered after a short illumination at 365 nm. Our results indicate that the accessibility of the protein machinery to one or several nucleic acid binding sites can be efficiently regulated by changing the conformational/condensation state of the nucleic acid (DNA conformation or mRNA aggregation), thus regulating gene activity in an efficient, reversible, and sequence-independent manner. The possibility offered by our approach to use light to trigger various gene expression systems in a system-independent way opens interesting perspectives to study gene expression dynamics as well as to develop photocontrolled biotechnological procedures.
DO - 10.1073/pnas.0904382106
UR - http://www.dx.doi.org/10.1073/pnas.0904382106
ER -