article · 01/01/2013
High-throughput and long-term observation of compartmentalized biochemical oscillators
Résumé
We report the splitting of an oscillating DNA circuit into similar to 700 droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenomena - slow desynchronization between the compartments and kinematic spatial waves - and investigate their possible origin. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.
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Hasatani, K., Leocmach, M., Genot, A.-J., Estevez-Torres, A., Fujii, T., & Rondelez, Y. (2013). High-throughput and long-term observation of compartmentalized biochemical oscillators. Chem. Commun., 49(73). https://doi.org/10.1039/c3cc44323j@article{Hasatani2013_91,
author = {Hasatani, Koshi and Leocmach, Mathieu and Genot, Anthony J. and Estevez-Torres, Andre and Fujii, Teruo and Rondelez, Yannick},
year = {2013},
month = {1},
title = {High-throughput and long-term observation of compartmentalized biochemical oscillators},
journal = {Chem. Commun.},
publisher = {ROYAL SOC CHEMISTRY},
volume = {49},
number = {73},
address = {THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND},
abstract = {We report the splitting of an oscillating DNA circuit into similar to 700 droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenomena - slow desynchronization between the compartments and kinematic spatial waves - and investigate their possible origin. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.},
url = {http://www.dx.doi.org/10.1039/c3cc44323j},
doi = {10.1039/c3cc44323j},
issn = {1359-7345},
}TY - JOUR
AU - Hasatani, Koshi
AU - Leocmach, Mathieu
AU - Genot, Anthony J.
AU - Estevez-Torres, Andre
AU - Fujii, Teruo
AU - Rondelez, Yannick
PY - 2013
DA - 2013/01/01
TI - High-throughput and long-term observation of compartmentalized biochemical oscillators
JO - Chem. Commun.
VL - 49
IS - 73
PB - ROYAL SOC CHEMISTRY
SN - 1359-7345
AB - We report the splitting of an oscillating DNA circuit into similar to 700 droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenomena - slow desynchronization between the compartments and kinematic spatial waves - and investigate their possible origin. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.
DO - 10.1039/c3cc44323j
UR - http://www.dx.doi.org/10.1039/c3cc44323j
ER -