article · 17/12/2015
Development of a real-time flexible multiphoton microendoscope for label-free imaging in a live animal
Résumé
We present a two-photon microendoscope capable of in vivo label-free deep-tissue high-resolution fast imaging through a very long optical fiber. First, an advanced light-pulse spectro-temporal shaping device optimally precompensates for linear and nonlinear distortions occurring during propagation within the endoscopic fiber. This enables the delivery of sub-40-fs duration infrared excitation pulses at the output of 5 meters of fiber. Second, the endoscopic fiber is a custom-made double-clad polarization-maintaining photonic crystal fiber specifically designed to optimize the imaging resolution and the intrinsic luminescence backward collection. Third, a miniaturized fiber-scanner of 2.2 mm outer diameter allows simultaneous second harmonic generation (SHG) and two-photon excited autofluorescence (TPEF) imaging at 8 frames per second. This microendoscope's transverse and axial resolutions amount respectively to 0.8 mu m and 12 mu m, with a field-of-view as large as 450 mu m. This microendoscope's unprecedented capabilities are validated during label-free imaging, ex vivo on various fixed human tissue samples, and in vivo on an anesthetized mouse kidney demonstrating an imaging penetration depth greater than 300 mu m below the surface of the organ. The results reported in this manuscript confirm that nonlinear microendoscopy can become a valuable clinical tool for real-time in situ assessment of pathological states.
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Ducourthial, G., Leclerc, P., Mansuryan, T., Fabert, M., Brevier, J., Habert, R., Braud, F., Batrin, R., Vever-Bizet, C., Bourg-Heckly, G., Thiberville, L., Druilhe, A., Kudlinski, A., & Louradour, F. (2015). Development of a real-time flexible multiphoton microendoscope for label-free imaging in a live animal. Sci Rep, 5(18303). https://doi.org/10.1038/srep18303@article{Ducourthial2015_80,
author = {Ducourthial, Guillaume and Leclerc, Pierre and Mansuryan, Tigran and Fabert, Marc and Brevier, Julien and Habert, Remi and Braud, Flavie and Batrin, Renaud and Vever-Bizet, Christine and Bourg-Heckly, Genevieve and Thiberville, Luc and Druilhe, Anne and Kudlinski, Alexandre and Louradour, Frederic},
year = {2015},
month = {12},
title = {Development of a real-time flexible multiphoton microendoscope for label-free imaging in a live animal},
journal = {Sci Rep},
publisher = {NATURE PUBLISHING GROUP},
volume = {5},
number = {18303},
address = {MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND},
abstract = {We present a two-photon microendoscope capable of in vivo label-free deep-tissue high-resolution fast imaging through a very long optical fiber. First, an advanced light-pulse spectro-temporal shaping device optimally precompensates for linear and nonlinear distortions occurring during propagation within the endoscopic fiber. This enables the delivery of sub-40-fs duration infrared excitation pulses at the output of 5 meters of fiber. Second, the endoscopic fiber is a custom-made double-clad polarization-maintaining photonic crystal fiber specifically designed to optimize the imaging resolution and the intrinsic luminescence backward collection. Third, a miniaturized fiber-scanner of 2.2 mm outer diameter allows simultaneous second harmonic generation (SHG) and two-photon excited autofluorescence (TPEF) imaging at 8 frames per second. This microendoscope's transverse and axial resolutions amount respectively to 0.8 mu m and 12 mu m, with a field-of-view as large as 450 mu m. This microendoscope's unprecedented capabilities are validated during label-free imaging, ex vivo on various fixed human tissue samples, and in vivo on an anesthetized mouse kidney demonstrating an imaging penetration depth greater than 300 mu m below the surface of the organ. The results reported in this manuscript confirm that nonlinear microendoscopy can become a valuable clinical tool for real-time in situ assessment of pathological states.},
url = {http://www.dx.doi.org/10.1038/srep18303},
doi = {10.1038/srep18303},
issn = {2045-2322},
}TY - JOUR
AU - Ducourthial, Guillaume
AU - Leclerc, Pierre
AU - Mansuryan, Tigran
AU - Fabert, Marc
AU - Brevier, Julien
AU - Habert, Remi
AU - Braud, Flavie
AU - Batrin, Renaud
AU - Vever-Bizet, Christine
AU - Bourg-Heckly, Genevieve
AU - Thiberville, Luc
AU - Druilhe, Anne
AU - Kudlinski, Alexandre
AU - Louradour, Frederic
PY - 2015
DA - 2015/12/17
TI - Development of a real-time flexible multiphoton microendoscope for label-free imaging in a live animal
JO - Sci Rep
VL - 5
IS - 18303
PB - NATURE PUBLISHING GROUP
SN - 2045-2322
AB - We present a two-photon microendoscope capable of in vivo label-free deep-tissue high-resolution fast imaging through a very long optical fiber. First, an advanced light-pulse spectro-temporal shaping device optimally precompensates for linear and nonlinear distortions occurring during propagation within the endoscopic fiber. This enables the delivery of sub-40-fs duration infrared excitation pulses at the output of 5 meters of fiber. Second, the endoscopic fiber is a custom-made double-clad polarization-maintaining photonic crystal fiber specifically designed to optimize the imaging resolution and the intrinsic luminescence backward collection. Third, a miniaturized fiber-scanner of 2.2 mm outer diameter allows simultaneous second harmonic generation (SHG) and two-photon excited autofluorescence (TPEF) imaging at 8 frames per second. This microendoscope's transverse and axial resolutions amount respectively to 0.8 mu m and 12 mu m, with a field-of-view as large as 450 mu m. This microendoscope's unprecedented capabilities are validated during label-free imaging, ex vivo on various fixed human tissue samples, and in vivo on an anesthetized mouse kidney demonstrating an imaging penetration depth greater than 300 mu m below the surface of the organ. The results reported in this manuscript confirm that nonlinear microendoscopy can become a valuable clinical tool for real-time in situ assessment of pathological states.
DO - 10.1038/srep18303
UR - http://www.dx.doi.org/10.1038/srep18303
ER -