article · 01/06/2016

ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems

Veronica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, Genevieve Bourg-Heckly, Anne Planat-Chretien

Résumé

We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).

Citer cet article

Sorgato, V., Berger, M., Emain, C., Vever-Bizet, C., Dinten, J.-M., Bourg-Heckly, G., & Planat-Chretien, A. (2016). ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems. J. Biomed. Opt., 21(6), 065003.1 - 065003.9. https://doi.org/10.1117/1.JBO.21.6.065003

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