article · 27/12/2013
Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity
Résumé
The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations ( cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact.
Citer cet article
Aubertin, K., Bonneau, S., Silva, A.-K.-A., Bacri, J.-C., Gallet, F., & Wilhelm, C. (2013). Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity. PLOS One, 8(12), UNSP e84850. https://doi.org/10.1371/journal.pone.0084850@article{Aubertin2013_56,
author = {Aubertin, Kelly and Bonneau, Stephanie and Silva, Amanda K. A. and Bacri, Jean-Claude and Gallet, Francois and Wilhelm, Claire},
year = {2013},
month = {12},
title = {Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity},
journal = {PLOS One},
volume = {8},
number = {12},
pages = {UNSP e84850},
abstract = {The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations ( cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact.},
url = {http://www.dx.doi.org/10.1371/journal.pone.0084850},
doi = {10.1371/journal.pone.0084850},
issn = {1932-6203},
}TY - JOUR
AU - Aubertin, Kelly
AU - Bonneau, Stephanie
AU - Silva, Amanda K. A.
AU - Bacri, Jean-Claude
AU - Gallet, Francois
AU - Wilhelm, Claire
PY - 2013
DA - 2013/12/27
TI - Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity
JO - PLOS One
VL - 8
IS - 12
SN - 1932-6203
AB - The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations ( cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact.
SP - UNSP e84850
DO - 10.1371/journal.pone.0084850
UR - http://www.dx.doi.org/10.1371/journal.pone.0084850
ER -