article · 01/04/2015
Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting
Résumé
Inspired by microvesicle-mediated intercellular communication, we propose a hybrid vector for magnetic drug delivery. It consists of macrophage-derived microvesicles engineered to enclose different therapeutic agents together with iron oxide nanoparticles. Here, we investigated in vitro how magnetic nanoparticles may influence the vector effectiveness in terms of drug uptake and targeting. Human macrophages were loaded with iron oxide nanoparticles and different therapeutic agents: a chemotherapeutic agent (doxorubicin), tissue-plasminogen activator (t-PA) and two photosensitizers (disulfonated tetraphenyl chlorin-TPCS2a and 5,10,15,20-tetra(m-hydroxyphenyl)chlorin-mTHPC). The hybrid cell microvesicles were magnetically responsive, readily manipulated by magnetic forces and MRI-detectable. Using photosensitizer-loaded vesicles, we showed that the uptake of microvesicles by cancer cells could be kinetically modulated and spatially controlled under magnetic field and that cancer cell death was enhanced by the magnetic targeting. From the Clinical Editor: In this article, the authors devised a biogenic method using macrophages to produce microvesicles containing both iron oxide and chemotherapeutic agents. They showed that the microvesicles could be manipulated by magnetic force for targeting and subsequent delivery of the drug payload against cancer cells. This smart method could provide a novel way for future fight against cancer. (C) 2015 Elsevier Inc. All rights reserved.
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Silva, A.-K.-A., Luciani, N., Gazeau, F., Aubertin, K., Bonneau, S., Chauvierre, C., Letourneur, D., & Wilhelm, C. (2015). Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting. Nanomedicine: NBM, 11(3), 645-655. https://doi.org/10.1016/j.nano.2014.11.009@article{Silva2015_55,
author = {Silva, Amanda K. A. and Luciani, Nathalie and Gazeau, Florence and Aubertin, Kelly and Bonneau, Stephanie and Chauvierre, Cedric and Letourneur, Didier and Wilhelm, Claire},
year = {2015},
month = {4},
title = {Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting},
journal = {Nanomedicine: NBM},
volume = {11},
number = {3},
pages = {645-655},
abstract = {Inspired by microvesicle-mediated intercellular communication, we propose a hybrid vector for magnetic drug delivery. It consists of macrophage-derived microvesicles engineered to enclose different therapeutic agents together with iron oxide nanoparticles. Here, we investigated in vitro how magnetic nanoparticles may influence the vector effectiveness in terms of drug uptake and targeting. Human macrophages were loaded with iron oxide nanoparticles and different therapeutic agents: a chemotherapeutic agent (doxorubicin), tissue-plasminogen activator (t-PA) and two photosensitizers (disulfonated tetraphenyl chlorin-TPCS2a and 5,10,15,20-tetra(m-hydroxyphenyl)chlorin-mTHPC). The hybrid cell microvesicles were magnetically responsive, readily manipulated by magnetic forces and MRI-detectable. Using photosensitizer-loaded vesicles, we showed that the uptake of microvesicles by cancer cells could be kinetically modulated and spatially controlled under magnetic field and that cancer cell death was enhanced by the magnetic targeting. From the Clinical Editor: In this article, the authors devised a biogenic method using macrophages to produce microvesicles containing both iron oxide and chemotherapeutic agents. They showed that the microvesicles could be manipulated by magnetic force for targeting and subsequent delivery of the drug payload against cancer cells. This smart method could provide a novel way for future fight against cancer. (C) 2015 Elsevier Inc. All rights reserved.},
url = {http://www.dx.doi.org/10.1016/j.nano.2014.11.009},
doi = {10.1016/j.nano.2014.11.009},
issn = {1549-9634},
}TY - JOUR
AU - Silva, Amanda K. A.
AU - Luciani, Nathalie
AU - Gazeau, Florence
AU - Aubertin, Kelly
AU - Bonneau, Stephanie
AU - Chauvierre, Cedric
AU - Letourneur, Didier
AU - Wilhelm, Claire
PY - 2015
DA - 2015/04/01
TI - Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting
JO - Nanomedicine: NBM
VL - 11
IS - 3
SN - 1549-9634
AB - Inspired by microvesicle-mediated intercellular communication, we propose a hybrid vector for magnetic drug delivery. It consists of macrophage-derived microvesicles engineered to enclose different therapeutic agents together with iron oxide nanoparticles. Here, we investigated in vitro how magnetic nanoparticles may influence the vector effectiveness in terms of drug uptake and targeting. Human macrophages were loaded with iron oxide nanoparticles and different therapeutic agents: a chemotherapeutic agent (doxorubicin), tissue-plasminogen activator (t-PA) and two photosensitizers (disulfonated tetraphenyl chlorin-TPCS2a and 5,10,15,20-tetra(m-hydroxyphenyl)chlorin-mTHPC). The hybrid cell microvesicles were magnetically responsive, readily manipulated by magnetic forces and MRI-detectable. Using photosensitizer-loaded vesicles, we showed that the uptake of microvesicles by cancer cells could be kinetically modulated and spatially controlled under magnetic field and that cancer cell death was enhanced by the magnetic targeting. From the Clinical Editor: In this article, the authors devised a biogenic method using macrophages to produce microvesicles containing both iron oxide and chemotherapeutic agents. They showed that the microvesicles could be manipulated by magnetic force for targeting and subsequent delivery of the drug payload against cancer cells. This smart method could provide a novel way for future fight against cancer. (C) 2015 Elsevier Inc. All rights reserved.
SP - 645
EP - 655
DO - 10.1016/j.nano.2014.11.009
UR - http://www.dx.doi.org/10.1016/j.nano.2014.11.009
ER -