Altered skeletal muscle mitochondrial biogenesis but improved endurance capacity in trained OPA1-deficient mice

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Caffin, F., Prola, A., Piquereau, J., Novotova, M., David, D.-J., Garnier, A., Fortin, D., Alavi, M.-V., Veksler, V., Ventura-Clapier, R., & Joubert, F. (2013). Altered skeletal muscle mitochondrial biogenesis but improved endurance capacity in trained OPA1-deficient mice. J. Physiol.-London, 591(23). https://doi.org/10.1113/jphysiol.2013.263079

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@article{Caffin2013_51,
  author = {Caffin, F. and Prola, A. and Piquereau, J. and Novotova, M. and David, D. J. and Garnier, A. and Fortin, D. and Alavi, M. V. and Veksler, V. and Ventura-Clapier, R. and Joubert, F.},
  year = {2013},
  month = {12},
  title = {Altered skeletal muscle mitochondrial biogenesis but improved endurance capacity in trained OPA1-deficient mice},
  journal = {J. Physiol.-London},
  publisher = {WILEY-BLACKWELL},
  volume = {591},
  number = {23},
  address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA},
  abstract = {The role of OPA1, a GTPase dynamin protein mainly involved in the fusion of inner mitochondrial membranes, has been studied in many cell types, but only a few studies have been conducted on adult differentiated tissues such as cardiac or skeletal muscle cells. Yet OPA1 is highly expressed in these cells, and could play different roles, especially in response to an environmental stress like exercise. Endurance exercise increases energy demand in skeletal muscle and repeated activity induces mitochondrial biogenesis and activation of fusion-fission cycles for the synthesis of new mitochondria. But currently no study has clearly shown a link between mitochondrial dynamics and biogenesis. Using a mouse model of haploinsufficiency for the Opa1 gene (Opa1(+/-)), we therefore studied the impact of OPA1 deficiency on the adaptation ability of fast skeletal muscles to endurance exercise training. Our results show that, surprisingly, Opa1(+/-) mice were able to perform the same physical activity as control mice. However, the adaptation strategies of both strains after training differed: while in control mice mitochondrial biogenesis was increased as expected, in Opa1(+/-) mice this process was blunted. Instead, training in Opa1(+/-) mice led to an increase in endurance capacity, and a specific adaptive response involving a metabolic remodelling towards enhanced fatty acid utilization. In conclusion, OPA1 appears necessary for the normal adaptive response and mitochondrial biogenesis of skeletal muscle to training. This work opens new perspectives on the role of mitochondrial dynamics in skeletal muscle cells and during adaptation to stress.},
  url = {http://www.dx.doi.org/10.1113/jphysiol.2013.263079},
  doi = {10.1113/jphysiol.2013.263079},
  issn = {0022-3751},
}

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TY  - JOUR
AU  - Caffin, F.
AU  - Prola, A.
AU  - Piquereau, J.
AU  - Novotova, M.
AU  - David, D. J.
AU  - Garnier, A.
AU  - Fortin, D.
AU  - Alavi, M. V.
AU  - Veksler, V.
AU  - Ventura-Clapier, R.
AU  - Joubert, F.
PY  - 2013
DA  - 2013/12/01
TI  - Altered skeletal muscle mitochondrial biogenesis but improved endurance capacity in trained OPA1-deficient mice
JO  - J. Physiol.-London
VL  - 591
IS  - 23
PB  - WILEY-BLACKWELL
SN  - 0022-3751
AB  - The role of OPA1, a GTPase dynamin protein mainly involved in the fusion of inner mitochondrial membranes, has been studied in many cell types, but only a few studies have been conducted on adult differentiated tissues such as cardiac or skeletal muscle cells. Yet OPA1 is highly expressed in these cells, and could play different roles, especially in response to an environmental stress like exercise. Endurance exercise increases energy demand in skeletal muscle and repeated activity induces mitochondrial biogenesis and activation of fusion-fission cycles for the synthesis of new mitochondria. But currently no study has clearly shown a link between mitochondrial dynamics and biogenesis. Using a mouse model of haploinsufficiency for the Opa1 gene (Opa1(+/-)), we therefore studied the impact of OPA1 deficiency on the adaptation ability of fast skeletal muscles to endurance exercise training. Our results show that, surprisingly, Opa1(+/-) mice were able to perform the same physical activity as control mice. However, the adaptation strategies of both strains after training differed: while in control mice mitochondrial biogenesis was increased as expected, in Opa1(+/-) mice this process was blunted. Instead, training in Opa1(+/-) mice led to an increase in endurance capacity, and a specific adaptive response involving a metabolic remodelling towards enhanced fatty acid utilization. In conclusion, OPA1 appears necessary for the normal adaptive response and mitochondrial biogenesis of skeletal muscle to training. This work opens new perspectives on the role of mitochondrial dynamics in skeletal muscle cells and during adaptation to stress.
DO  - 10.1113/jphysiol.2013.263079
UR  - http://www.dx.doi.org/10.1113/jphysiol.2013.263079
ER  -