article · 27/03/2024
Distinct and Asymmetric Neuronal Responses to Pitch- and Roll-Axis Vestibular Stimulation in larval zebrafish
Résumé
The vestibular apparatus plays a pivotal role in maintaining postural equilibrium and processing movement signals, underscoring its importance in the study of certain neurological disorders. Here, we present a rotating light-sheet microscope, enabling brain-wide functional recordings during dynamic vestibular stimulation along both the roll and pitch axes in head-restrained zebrafish larvae. The system incorporates a double galvanometer mirror configuration, is amenable to 3D printing, and enhances scanning efficiency. Employing this apparatus, we have successfully conducted the first comprehensive mapping of zebrafish brain responses to dynamic pitch-tilt vestibular stimulation. Through Fourier and regression analyses, we report an asymmetry in neuronal recruitment during nose-up versus nose-down pitch tilts within critical regions including the cerebellum, oculomotor nucleus, caudal hindbrain, and vestibular nucleus, highlighting physiological adaptations to downward motion. We identified specific brain regions, notably the cerebellum and medial-rostral rhombencephalon, that exclusively respond to rollbut not pitch-tilt vestibular stimuli. Furthermore, we have identified a transgenic line that closely correlates with our functional mappings and demonstrates a significant response to vestibular stimulation. The elucidation of brain-wide neuronal circuits involved in vestibular processing establishes a foundational framework for subsequent detailed investigations into the molecular and genetic mechanisms underlying postural control and motion perception.
Citer cet article
Migault, G., Beiza-Canelo, N., Chatterjee, S., Debrégeas, G., & Bormuth, V. (2024). Distinct and Asymmetric Neuronal Responses to Pitch- and Roll-Axis Vestibular Stimulation in larval zebrafish. bioRxiv. https://doi.org/doi: https://doi.org/10.1101/2024.03.22.586054@article{Migault2024_473,
author = {Migault, Geoffrey and Beiza-Canelo, Natalia and Chatterjee, Sharbatanu and Debrégeas, Georges and Bormuth, Volker},
year = {2024},
month = {3},
title = {Distinct and Asymmetric Neuronal Responses to Pitch- and Roll-Axis Vestibular Stimulation in larval zebrafish},
journal = {bioRxiv},
abstract = {The vestibular apparatus plays a pivotal role in maintaining postural equilibrium and processing movement signals, underscoring its importance in the study of certain neurological disorders. Here, we present a rotating light-sheet microscope, enabling brain-wide functional recordings during dynamic vestibular stimulation along both the roll and pitch axes in head-restrained zebrafish larvae. The system incorporates a double galvanometer mirror configuration, is amenable to 3D printing, and enhances scanning efficiency. Employing this apparatus, we have successfully conducted the first comprehensive mapping of zebrafish brain responses to dynamic pitch-tilt vestibular stimulation. Through Fourier and regression analyses, we report an asymmetry in neuronal recruitment during nose-up versus nose-down pitch tilts within critical regions including the cerebellum, oculomotor nucleus, caudal hindbrain, and vestibular nucleus, highlighting physiological adaptations to downward motion. We identified specific brain regions, notably the cerebellum and medial-rostral rhombencephalon, that exclusively respond to rollbut not pitch-tilt vestibular stimuli. Furthermore, we have identified a transgenic line that closely correlates with our functional mappings and demonstrates a significant response to vestibular stimulation. The elucidation of brain-wide neuronal circuits involved in vestibular processing establishes a foundational framework for subsequent detailed investigations into the molecular and genetic mechanisms underlying postural control and motion perception.},
url = {https://www.biorxiv.org/content/10.1101/2024.03.22.586054v1},
doi = {doi: https://doi.org/10.1101/2024.03.22.586054},
}TY - JOUR
AU - Migault, Geoffrey
AU - Beiza-Canelo, Natalia
AU - Chatterjee, Sharbatanu
AU - Debrégeas, Georges
AU - Bormuth, Volker
PY - 2024
DA - 2024/03/27
TI - Distinct and Asymmetric Neuronal Responses to Pitch- and Roll-Axis Vestibular Stimulation in larval zebrafish
JO - bioRxiv
AB - The vestibular apparatus plays a pivotal role in maintaining postural equilibrium and processing movement signals, underscoring its importance in the study of certain neurological disorders. Here, we present a rotating light-sheet microscope, enabling brain-wide functional recordings during dynamic vestibular stimulation along both the roll and pitch axes in head-restrained zebrafish larvae. The system incorporates a double galvanometer mirror configuration, is amenable to 3D printing, and enhances scanning efficiency. Employing this apparatus, we have successfully conducted the first comprehensive mapping of zebrafish brain responses to dynamic pitch-tilt vestibular stimulation. Through Fourier and regression analyses, we report an asymmetry in neuronal recruitment during nose-up versus nose-down pitch tilts within critical regions including the cerebellum, oculomotor nucleus, caudal hindbrain, and vestibular nucleus, highlighting physiological adaptations to downward motion. We identified specific brain regions, notably the cerebellum and medial-rostral rhombencephalon, that exclusively respond to rollbut not pitch-tilt vestibular stimuli. Furthermore, we have identified a transgenic line that closely correlates with our functional mappings and demonstrates a significant response to vestibular stimulation. The elucidation of brain-wide neuronal circuits involved in vestibular processing establishes a foundational framework for subsequent detailed investigations into the molecular and genetic mechanisms underlying postural control and motion perception.
DO - doi: https://doi.org/10.1101/2024.03.22.586054
UR - https://www.biorxiv.org/content/10.1101/2024.03.22.586054v1
ER -