article · 01/12/2014
Effect of PKC alpha expression on Bcl-2 phosphorylation and cell death by hypericin
Résumé
In order to explain the contribution of the protein kinase C alpha (PKC alpha) in apoptosis induced by photo-activation of hypericin (Hyp), a small interfering RNA was used for post-transcriptional silencing of pkc alpha gene expression. We have evaluated the influence of Hyp photo-activation on cell death in non-transfected and transfected (PKC alpha(-)) human glioma cells (U-87 MG). No significant differences were detected in cell survival between non-transfected and transfected PKC alpha(-) cells. However, the type of cell death was notably affected by silencing the pkc alpha gene. Photo-activation of Hyp strongly induced apoptosis in non-transfected cells, but the level of necrotic cells in transfected PKC alpha(-) cells increased significantly. The differences in cell death after Hyp photo-activation are demonstrated by changes in: (i) reactive oxygen species production, (ii) Bcl-2 phosphorylation on Ser70 (pBcl-2(Ser70)), (iii) cellular distributions of pBcl-2(Ser70) and (iv) cellular distribution of endogenous anti-oxidant glutathione and its co-localization with mitochondria. In summary, we suggest that post-transcriptional silencing of the pkc alpha gene and the related decrease of PKC alpha level considerably affects the anti-apoptotic function and the anti-oxidant function of Bcl-2. This implies that PKC alpha, as Bcl-2 kinase, indirectly protects U-87 MG cells against oxidative stress and subsequent cell death.
Citer cet article
Joniova, J., Misuth, M., Sureau, F., Miskovsky, P., & Nadova, Z. (2014). Effect of PKC alpha expression on Bcl-2 phosphorylation and cell death by hypericin. Apoptosis, 19(12), 1779-1792. https://doi.org/10.1007/s10495-014-1043-7@article{Joniova2014_46,
author = {Joniova, Jaroslava and Misuth, Matus and Sureau, Franck and Miskovsky, Pavol and Nadova, Zuzana},
year = {2014},
month = {12},
title = {Effect of PKC alpha expression on Bcl-2 phosphorylation and cell death by hypericin},
journal = {Apoptosis},
publisher = {SPRINGER},
volume = {19},
number = {12},
pages = {1779-1792},
address = {VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS},
abstract = {In order to explain the contribution of the protein kinase C alpha (PKC alpha) in apoptosis induced by photo-activation of hypericin (Hyp), a small interfering RNA was used for post-transcriptional silencing of pkc alpha gene expression. We have evaluated the influence of Hyp photo-activation on cell death in non-transfected and transfected (PKC alpha(-)) human glioma cells (U-87 MG). No significant differences were detected in cell survival between non-transfected and transfected PKC alpha(-) cells. However, the type of cell death was notably affected by silencing the pkc alpha gene. Photo-activation of Hyp strongly induced apoptosis in non-transfected cells, but the level of necrotic cells in transfected PKC alpha(-) cells increased significantly. The differences in cell death after Hyp photo-activation are demonstrated by changes in: (i) reactive oxygen species production, (ii) Bcl-2 phosphorylation on Ser70 (pBcl-2(Ser70)), (iii) cellular distributions of pBcl-2(Ser70) and (iv) cellular distribution of endogenous anti-oxidant glutathione and its co-localization with mitochondria. In summary, we suggest that post-transcriptional silencing of the pkc alpha gene and the related decrease of PKC alpha level considerably affects the anti-apoptotic function and the anti-oxidant function of Bcl-2. This implies that PKC alpha, as Bcl-2 kinase, indirectly protects U-87 MG cells against oxidative stress and subsequent cell death.},
url = {http://www.dx.doi.org/10.1007/s10495-014-1043-7},
doi = {10.1007/s10495-014-1043-7},
issn = {1360-8185},
}TY - JOUR
AU - Joniova, Jaroslava
AU - Misuth, Matus
AU - Sureau, Franck
AU - Miskovsky, Pavol
AU - Nadova, Zuzana
PY - 2014
DA - 2014/12/01
TI - Effect of PKC alpha expression on Bcl-2 phosphorylation and cell death by hypericin
JO - Apoptosis
VL - 19
IS - 12
PB - SPRINGER
SN - 1360-8185
AB - In order to explain the contribution of the protein kinase C alpha (PKC alpha) in apoptosis induced by photo-activation of hypericin (Hyp), a small interfering RNA was used for post-transcriptional silencing of pkc alpha gene expression. We have evaluated the influence of Hyp photo-activation on cell death in non-transfected and transfected (PKC alpha(-)) human glioma cells (U-87 MG). No significant differences were detected in cell survival between non-transfected and transfected PKC alpha(-) cells. However, the type of cell death was notably affected by silencing the pkc alpha gene. Photo-activation of Hyp strongly induced apoptosis in non-transfected cells, but the level of necrotic cells in transfected PKC alpha(-) cells increased significantly. The differences in cell death after Hyp photo-activation are demonstrated by changes in: (i) reactive oxygen species production, (ii) Bcl-2 phosphorylation on Ser70 (pBcl-2(Ser70)), (iii) cellular distributions of pBcl-2(Ser70) and (iv) cellular distribution of endogenous anti-oxidant glutathione and its co-localization with mitochondria. In summary, we suggest that post-transcriptional silencing of the pkc alpha gene and the related decrease of PKC alpha level considerably affects the anti-apoptotic function and the anti-oxidant function of Bcl-2. This implies that PKC alpha, as Bcl-2 kinase, indirectly protects U-87 MG cells against oxidative stress and subsequent cell death.
SP - 1779
EP - 1792
DO - 10.1007/s10495-014-1043-7
UR - http://www.dx.doi.org/10.1007/s10495-014-1043-7
ER -