article · 24/02/2024
Lipidomics and biodistribution of extracellular vesicles-secreted by hepatocytes from Zucker lean and fatty rats
Abstract
Extracellular vesicles (EVs) have been involved in metabolic syndrome, although their specific role in the development of the pathology is still unknown. To further study the role of EVs, we have analysed by Raman tweezers microspectroscopy and mass spectrometry-based lipidomics the small EVs population secreted by fatty (ZF) and lean (ZL) hepatocytes obtained from Zucker rats. We have also explored in vivo and ex vivo biodistribution of these EVs through fluorine-18-radiolabelling using a positron emission tomography imaging. Based on the proportion of proteins to lipids and the types of lipids, our results indicate that within the range of small EVs, primary hepatocytes secrete different subpopulations of particles. These differences were observed in the enrichment of triglyceride species in EVs secreted by ZF hepatocytes. Biodistribution experiments showed accumulation in the brain, heart, lungs, kidney and specially in bladder after intravenous administration. In summary, we show that EVs released by a fatty hepatocytes carry a different lipid signature compared to their lean counterpart. Biodistribution experiment has shown no difference in the distribution of EVs secreted by ZF and ZL hepatocytes but has given us a first view of possible target organs for these particles. Our results might open a door to both pathology studies and therapeutic interventions.
Cite this article
Azparren-Angulo, M., Mleczko, J., Alboniga, O.-E., Kruglik, S.-G., Guigner, J.-M., Gonzalez, E., Garcia-Vallicrosa, C., Llop, J., Simó, C., Alonso, C., Iruarrizaga, M., Royo, F., & Falcon-Perez, J.-M. (2024). Lipidomics and biodistribution of extracellular vesicles-secreted by hepatocytes from Zucker lean and fatty rats. Journal of Extracellular Biology, 3(2). https://doi.org/10.1002/jex2.140@article{AzparrenAngulo2024_455,
author = {Azparren-Angulo, Maria and Mleczko, Justyna and Alboniga, Oihane E. and Kruglik, Sergei G. and Guigner, Jean-Michel and Gonzalez, Esperanza and Garcia-Vallicrosa, Clara and Llop, Jordi and Simó, Cristina and Alonso, Cristina and Iruarrizaga, Marta and Royo, Felix and Falcon-Perez, Juan M.},
year = {2024},
month = {2},
title = {Lipidomics and biodistribution of extracellular vesicles-secreted by hepatocytes from Zucker lean and fatty rats},
journal = {Journal of Extracellular Biology},
volume = {3},
number = {2},
abstract = {Extracellular vesicles (EVs) have been involved in metabolic syndrome, although their specific role in the development of the pathology is still unknown. To further study the role of EVs, we have analysed by Raman tweezers microspectroscopy and mass spectrometry-based lipidomics the small EVs population secreted by fatty (ZF) and lean (ZL) hepatocytes obtained from Zucker rats. We have also explored in vivo and ex vivo biodistribution of these EVs through fluorine-18-radiolabelling using a positron emission tomography imaging. Based on the proportion of proteins to lipids and the types of lipids, our results indicate that within the range of small EVs, primary hepatocytes secrete different subpopulations of particles. These differences were observed in the enrichment of triglyceride species in EVs secreted by ZF hepatocytes. Biodistribution experiments showed accumulation in the brain, heart, lungs, kidney and specially in bladder after intravenous administration. In summary, we show that EVs released by a fatty hepatocytes carry a different lipid signature compared to their lean counterpart. Biodistribution experiment has shown no difference in the distribution of EVs secreted by ZF and ZL hepatocytes but has given us a first view of possible target organs for these particles. Our results might open a door to both pathology studies and therapeutic interventions.},
url = {https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jex2.140},
doi = {10.1002/jex2.140},
}TY - JOUR
AU - Azparren-Angulo, Maria
AU - Mleczko, Justyna
AU - Alboniga, Oihane E.
AU - Kruglik, Sergei G.
AU - Guigner, Jean-Michel
AU - Gonzalez, Esperanza
AU - Garcia-Vallicrosa, Clara
AU - Llop, Jordi
AU - Simó, Cristina
AU - Alonso, Cristina
AU - Iruarrizaga, Marta
AU - Royo, Felix
AU - Falcon-Perez, Juan M.
PY - 2024
DA - 2024/02/24
TI - Lipidomics and biodistribution of extracellular vesicles-secreted by hepatocytes from Zucker lean and fatty rats
JO - Journal of Extracellular Biology
VL - 3
IS - 2
AB - Extracellular vesicles (EVs) have been involved in metabolic syndrome, although their specific role in the development of the pathology is still unknown. To further study the role of EVs, we have analysed by Raman tweezers microspectroscopy and mass spectrometry-based lipidomics the small EVs population secreted by fatty (ZF) and lean (ZL) hepatocytes obtained from Zucker rats. We have also explored in vivo and ex vivo biodistribution of these EVs through fluorine-18-radiolabelling using a positron emission tomography imaging. Based on the proportion of proteins to lipids and the types of lipids, our results indicate that within the range of small EVs, primary hepatocytes secrete different subpopulations of particles. These differences were observed in the enrichment of triglyceride species in EVs secreted by ZF hepatocytes. Biodistribution experiments showed accumulation in the brain, heart, lungs, kidney and specially in bladder after intravenous administration. In summary, we show that EVs released by a fatty hepatocytes carry a different lipid signature compared to their lean counterpart. Biodistribution experiment has shown no difference in the distribution of EVs secreted by ZF and ZL hepatocytes but has given us a first view of possible target organs for these particles. Our results might open a door to both pathology studies and therapeutic interventions.
DO - 10.1002/jex2.140
UR - https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jex2.140
ER -