Dynamics of mitochondrial under oxidative stress with high spatiotemporal resolution

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Loriette, V., Fragola, A., Kruglik, S., Sridhar, S., Hubert, A., Orieux, F., Sepulveda, E., Sureau, F., & Bonneau, S. (2023). Dynamics of mitochondrial under oxidative stress with high spatiotemporal resolution. Front. Cell Dev. Biol., 11. https://doi.org/https://doi.org/10.3389/fcell.2023.1307502

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@article{Loriette2023_451,
  author = {Loriette, Vincent and Fragola, Alexandra and Kruglik, Sergei and Sridhar, Susmita and Hubert, Antoine and Orieux, François and Sepulveda, Eduardo and Sureau, Franck and Bonneau, Stephanie},
  year = {2023},
  month = {11},
  title = {Dynamics of mitochondrial under oxidative stress with high spatiotemporal resolution},
  journal = {Front. Cell Dev. Biol.},
  volume = {11},
  abstract = {In our study, we harnessed an original Enhanced Speed Structured Illumination Microscopy (Fast-SIM) imaging setup to explore the dynamics of mitochondrial and inner membrane ultrastructure under specific photo-oxidation stress induced by Chlorin-e6 and light irradiation. Notably, our Fast-SIM system allowed us to observe and quantify a distinct remodeling and shortening of the mitochondrial structure after 60–80 s of irradiation. These changes were accompanied by fusion events of adjacent inner membrane cristae and global swelling of the organelle. Preceding these alterations, a larger sequence was characterized by heightened dynamics within the mitochondrial network, featuring events such as mitochondrial fission, rapid formation of tubular prolongations, and fluctuations in cristae structure. Our findings provide compelling evidence that, among enhanced-resolution microscopy techniques, Fast-SIM emerges as the most suitable approach for non-invasive dynamic studies of mitochondrial structure in living cells. For the first time, this approach allows quantitative and qualitative characterization of successive steps in the photo-induced oxidation process with sufficient spatial and temporal resolution.},
  doi = {https://doi.org/10.3389/fcell.2023.1307502},
}

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TY  - JOUR
AU  - Loriette, Vincent
AU  - Fragola, Alexandra
AU  - Kruglik, Sergei
AU  - Sridhar, Susmita
AU  - Hubert, Antoine
AU  - Orieux, François
AU  - Sepulveda, Eduardo
AU  - Sureau, Franck
AU  - Bonneau, Stephanie
PY  - 2023
DA  - 2023/11/17
TI  - Dynamics of mitochondrial under oxidative stress with high spatiotemporal resolution
JO  - Front. Cell Dev. Biol.
VL  - 11
AB  - In our study, we harnessed an original Enhanced Speed Structured Illumination Microscopy (Fast-SIM) imaging setup to explore the dynamics of mitochondrial and inner membrane ultrastructure under specific photo-oxidation stress induced by Chlorin-e6 and light irradiation. Notably, our Fast-SIM system allowed us to observe and quantify a distinct remodeling and shortening of the mitochondrial structure after 60–80 s of irradiation. These changes were accompanied by fusion events of adjacent inner membrane cristae and global swelling of the organelle. Preceding these alterations, a larger sequence was characterized by heightened dynamics within the mitochondrial network, featuring events such as mitochondrial fission, rapid formation of tubular prolongations, and fluctuations in cristae structure. Our findings provide compelling evidence that, among enhanced-resolution microscopy techniques, Fast-SIM emerges as the most suitable approach for non-invasive dynamic studies of mitochondrial structure in living cells. For the first time, this approach allows quantitative and qualitative characterization of successive steps in the photo-induced oxidation process with sufficient spatial and temporal resolution.
DO  - https://doi.org/10.3389/fcell.2023.1307502
ER  -