article · 01/09/2015
Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging
Résumé
Time-resolved microspectrofluorimetry and fluorescence microscopy imaging-two complementary fluorescence techniques-provide important information about the intracellular distribution, level of uptake and binding/interactions inside living cell of the labeled molecule of interest. They were employed to monitor the ``fate{''} of AS1411 aptamer labeled by ATTO 425 in human living cells. Confocal microspectrofluorimeter adapted for time-resolved intracellular fluorescence measurements by using a phase-modulation principle with homodyne data acquisition was employed to obtain emission spectra and to determine fluorescence lifetimes in U-87 MG tumor brain cells and Hs68 non-tumor foreskin cells. Acquired spectra from both the intracellular space and the reference solutions were treated to observe the aptamer localization and its interaction with biological structures inside the living cell. The emission spectra and the maximum emission wavelengths coming from the cells are practically identical, however significant lifetime lengthening was observed for tumor cell line in comparison to non-tumor one.
Citer cet article
Kocisova, E., Praus, P., Bok, J., Bonneau, S., & Sureau, F. (2015). Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging. J. Fluoresc., 25(5), 1245-1250. https://doi.org/10.1007/s10895-015-1612-3@article{Kocisova2015_45,
author = {Kocisova, Eva and Praus, Petr and Bok, Jiri and Bonneau, Stephanie and Sureau, Franck},
year = {2015},
month = {9},
title = {Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging},
journal = {J. Fluoresc.},
publisher = {SPRINGER/PLENUM PUBLISHERS},
volume = {25},
number = {5},
pages = {1245-1250},
address = {233 SPRING ST, NEW YORK, NY 10013 USA},
abstract = {Time-resolved microspectrofluorimetry and fluorescence microscopy imaging-two complementary fluorescence techniques-provide important information about the intracellular distribution, level of uptake and binding/interactions inside living cell of the labeled molecule of interest. They were employed to monitor the ``fate\{''\} of AS1411 aptamer labeled by ATTO 425 in human living cells. Confocal microspectrofluorimeter adapted for time-resolved intracellular fluorescence measurements by using a phase-modulation principle with homodyne data acquisition was employed to obtain emission spectra and to determine fluorescence lifetimes in U-87 MG tumor brain cells and Hs68 non-tumor foreskin cells. Acquired spectra from both the intracellular space and the reference solutions were treated to observe the aptamer localization and its interaction with biological structures inside the living cell. The emission spectra and the maximum emission wavelengths coming from the cells are practically identical, however significant lifetime lengthening was observed for tumor cell line in comparison to non-tumor one.},
url = {http://www.dx.doi.org/10.1007/s10895-015-1612-3},
doi = {10.1007/s10895-015-1612-3},
issn = {1053-0509},
}TY - JOUR
AU - Kocisova, Eva
AU - Praus, Petr
AU - Bok, Jiri
AU - Bonneau, Stephanie
AU - Sureau, Franck
PY - 2015
DA - 2015/09/01
TI - Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging
JO - J. Fluoresc.
VL - 25
IS - 5
PB - SPRINGER/PLENUM PUBLISHERS
SN - 1053-0509
AB - Time-resolved microspectrofluorimetry and fluorescence microscopy imaging-two complementary fluorescence techniques-provide important information about the intracellular distribution, level of uptake and binding/interactions inside living cell of the labeled molecule of interest. They were employed to monitor the ``fate{''} of AS1411 aptamer labeled by ATTO 425 in human living cells. Confocal microspectrofluorimeter adapted for time-resolved intracellular fluorescence measurements by using a phase-modulation principle with homodyne data acquisition was employed to obtain emission spectra and to determine fluorescence lifetimes in U-87 MG tumor brain cells and Hs68 non-tumor foreskin cells. Acquired spectra from both the intracellular space and the reference solutions were treated to observe the aptamer localization and its interaction with biological structures inside the living cell. The emission spectra and the maximum emission wavelengths coming from the cells are practically identical, however significant lifetime lengthening was observed for tumor cell line in comparison to non-tumor one.
SP - 1245
EP - 1250
DO - 10.1007/s10895-015-1612-3
UR - http://www.dx.doi.org/10.1007/s10895-015-1612-3
ER -