article · 20/08/2016
Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins
Résumé
Recent years have seen a growing interest in Berberine, a phytochemical with multispectrum therapeutic activities, as anti-tumoral agent for photodynamic therapy (PDT). In this context, low density lipoproteins (LDL) play a key role in the delivery of the photosensitizer in tumor cells. We correlate the physicochemical parameters of the berberine association to LDL with the influence of LDL-delivery on its accumulation in a glioma cell line and on its photo-induced activity in view of antitumor PDT. Our results evidence an important binding of 400 berberine molecules per LDL. Changes in berberine and apoprotein fluorescence suggest different fixation types, involving various LDL compartments including the vicinity of the apoprotein. The berberine association to LDL does not affect their recognition by the specific B/E receptors, of which over-expression increases the cellular uptake of LDL-preloaded berberine. Fluorescence microscopy evidences the mitochondrial labeling of the glioma model cells, with no significant modification upon LDL-delivery. Moreover, the cellular delivery of berberine by LDL increases its photocytotoxic effects on such cells. So, this research illustrates the potential of berberine as a photosensitizing agent for PDT, in particular due to their behavior towards LDL as plasma vehicles, and gives insights into its mechanisms of cell uptake. (C) 2016 Published by Elsevier B.V.
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Andreazza, N.-L., Vevert-Bizet, C., Bourg-Heckly, G., Sureau, F., Salvador, M.-J., & Bonneau, S. (2016). Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins. Int. J. Pharm., 510(1), 240-249. https://doi.org/10.1016/j.ijpharm.2016.06.009@article{Andreazza2016_43,
author = {Andreazza, Nathalia Luiza and Vevert-Bizet, Christine and Bourg-Heckly, Genevieve and Sureau, Franck and Salvador, Marcos Jose and Bonneau, Stephanie},
year = {2016},
month = {8},
title = {Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins},
journal = {Int. J. Pharm.},
publisher = {ELSEVIER SCIENCE BV},
volume = {510},
number = {1},
pages = {240-249},
address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
abstract = {Recent years have seen a growing interest in Berberine, a phytochemical with multispectrum therapeutic activities, as anti-tumoral agent for photodynamic therapy (PDT). In this context, low density lipoproteins (LDL) play a key role in the delivery of the photosensitizer in tumor cells. We correlate the physicochemical parameters of the berberine association to LDL with the influence of LDL-delivery on its accumulation in a glioma cell line and on its photo-induced activity in view of antitumor PDT. Our results evidence an important binding of 400 berberine molecules per LDL. Changes in berberine and apoprotein fluorescence suggest different fixation types, involving various LDL compartments including the vicinity of the apoprotein. The berberine association to LDL does not affect their recognition by the specific B/E receptors, of which over-expression increases the cellular uptake of LDL-preloaded berberine. Fluorescence microscopy evidences the mitochondrial labeling of the glioma model cells, with no significant modification upon LDL-delivery. Moreover, the cellular delivery of berberine by LDL increases its photocytotoxic effects on such cells. So, this research illustrates the potential of berberine as a photosensitizing agent for PDT, in particular due to their behavior towards LDL as plasma vehicles, and gives insights into its mechanisms of cell uptake. (C) 2016 Published by Elsevier B.V.},
url = {http://www.dx.doi.org/10.1016/j.ijpharm.2016.06.009},
doi = {10.1016/j.ijpharm.2016.06.009},
issn = {0378-5173},
}TY - JOUR
AU - Andreazza, Nathalia Luiza
AU - Vevert-Bizet, Christine
AU - Bourg-Heckly, Genevieve
AU - Sureau, Franck
AU - Salvador, Marcos Jose
AU - Bonneau, Stephanie
PY - 2016
DA - 2016/08/20
TI - Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins
JO - Int. J. Pharm.
VL - 510
IS - 1
PB - ELSEVIER SCIENCE BV
SN - 0378-5173
AB - Recent years have seen a growing interest in Berberine, a phytochemical with multispectrum therapeutic activities, as anti-tumoral agent for photodynamic therapy (PDT). In this context, low density lipoproteins (LDL) play a key role in the delivery of the photosensitizer in tumor cells. We correlate the physicochemical parameters of the berberine association to LDL with the influence of LDL-delivery on its accumulation in a glioma cell line and on its photo-induced activity in view of antitumor PDT. Our results evidence an important binding of 400 berberine molecules per LDL. Changes in berberine and apoprotein fluorescence suggest different fixation types, involving various LDL compartments including the vicinity of the apoprotein. The berberine association to LDL does not affect their recognition by the specific B/E receptors, of which over-expression increases the cellular uptake of LDL-preloaded berberine. Fluorescence microscopy evidences the mitochondrial labeling of the glioma model cells, with no significant modification upon LDL-delivery. Moreover, the cellular delivery of berberine by LDL increases its photocytotoxic effects on such cells. So, this research illustrates the potential of berberine as a photosensitizing agent for PDT, in particular due to their behavior towards LDL as plasma vehicles, and gives insights into its mechanisms of cell uptake. (C) 2016 Published by Elsevier B.V.
SP - 240
EP - 249
DO - 10.1016/j.ijpharm.2016.06.009
UR - http://www.dx.doi.org/10.1016/j.ijpharm.2016.06.009
ER -