article · 01/10/2009
Nanovesicles released by Dictyostelium cells: A potential carrier for drug delivery
Résumé
Nanovesicles released by Dictyostelium discoideum cells grown in the presence of the DNA-specific dye Hoechst 33342 have been previously shown to mediate the transfer of the dye into the nuclei of Hoechst-resistant cells. The present investigation extends this work by conducting experiments in the presence of hypericin, a fluorescent therapeutic photosensitizer assayed for antitumoral photodynamic therapy. Nanovesicles released by Dictyostelium cells exhibit an averaged diameter between 50 and 150 nm, as measured by transmission cryoelectron microscopy. A proteomic analysis reveals a predominance of actin and actin-related proteins. The detection of a lysosomal membrane protein (LIMP II) indicates that these vesicles are likely generated in the late endosomal compartment. The use of the hypericin-containing nanovesicles as nanodevices for in vitro drug delivery was investigated by fluorescence microscopy. The observed signal was almost exclusively located in the perinuclear area of two human cell lines, skin fibroblasts (HS68)and cervix carcinoma (HeLa)cells. Studies by confocal microscopy with specific markers of cell organelles, provided evidence that hypericin was accumulated in the Golgi apparatus. All these data shed a new light on in vitro drug delivery by using cell-released vesicles as carriers. (C) 2009 Elsevier B.V. All rights reserved.
Citer cet article
Lavialle, F., Deshayes, S., Gonnet, F., Larquet, E., Kruglik, S.-G., Boisset, N., Daniel, R., Alfsen, A., & Tatischeff, I. (2009). Nanovesicles released by Dictyostelium cells: A potential carrier for drug delivery. International Journal of Pharmaceutics, 380(1-2), 206-215. https://doi.org/10.1016/j.ijpharm.2009.06.039@article{Lavialle2009_40,
author = {Lavialle, Francoise and Deshayes, Sophie and Gonnet, Florence and Larquet, Eric and Kruglik, Sergei G. and Boisset, Nicolas and Daniel, Regis and Alfsen, Annette and Tatischeff, Irene},
year = {2009},
month = {10},
title = {Nanovesicles released by Dictyostelium cells: A potential carrier for drug delivery},
journal = {International Journal of Pharmaceutics},
publisher = {ELSEVIER SCIENCE BV},
volume = {380},
number = {1-2},
pages = {206-215},
address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
abstract = {Nanovesicles released by Dictyostelium discoideum cells grown in the presence of the DNA-specific dye Hoechst 33342 have been previously shown to mediate the transfer of the dye into the nuclei of Hoechst-resistant cells. The present investigation extends this work by conducting experiments in the presence of hypericin, a fluorescent therapeutic photosensitizer assayed for antitumoral photodynamic therapy. Nanovesicles released by Dictyostelium cells exhibit an averaged diameter between 50 and 150 nm, as measured by transmission cryoelectron microscopy. A proteomic analysis reveals a predominance of actin and actin-related proteins. The detection of a lysosomal membrane protein (LIMP II) indicates that these vesicles are likely generated in the late endosomal compartment. The use of the hypericin-containing nanovesicles as nanodevices for in vitro drug delivery was investigated by fluorescence microscopy. The observed signal was almost exclusively located in the perinuclear area of two human cell lines, skin fibroblasts (HS68)and cervix carcinoma (HeLa)cells. Studies by confocal microscopy with specific markers of cell organelles, provided evidence that hypericin was accumulated in the Golgi apparatus. All these data shed a new light on in vitro drug delivery by using cell-released vesicles as carriers. (C) 2009 Elsevier B.V. All rights reserved.},
url = {http://www.dx.doi.org/10.1016/j.ijpharm.2009.06.039},
doi = {10.1016/j.ijpharm.2009.06.039},
issn = {0378-5173},
}TY - JOUR
AU - Lavialle, Francoise
AU - Deshayes, Sophie
AU - Gonnet, Florence
AU - Larquet, Eric
AU - Kruglik, Sergei G.
AU - Boisset, Nicolas
AU - Daniel, Regis
AU - Alfsen, Annette
AU - Tatischeff, Irene
PY - 2009
DA - 2009/10/01
TI - Nanovesicles released by Dictyostelium cells: A potential carrier for drug delivery
JO - International Journal of Pharmaceutics
VL - 380
IS - 1-2
PB - ELSEVIER SCIENCE BV
SN - 0378-5173
AB - Nanovesicles released by Dictyostelium discoideum cells grown in the presence of the DNA-specific dye Hoechst 33342 have been previously shown to mediate the transfer of the dye into the nuclei of Hoechst-resistant cells. The present investigation extends this work by conducting experiments in the presence of hypericin, a fluorescent therapeutic photosensitizer assayed for antitumoral photodynamic therapy. Nanovesicles released by Dictyostelium cells exhibit an averaged diameter between 50 and 150 nm, as measured by transmission cryoelectron microscopy. A proteomic analysis reveals a predominance of actin and actin-related proteins. The detection of a lysosomal membrane protein (LIMP II) indicates that these vesicles are likely generated in the late endosomal compartment. The use of the hypericin-containing nanovesicles as nanodevices for in vitro drug delivery was investigated by fluorescence microscopy. The observed signal was almost exclusively located in the perinuclear area of two human cell lines, skin fibroblasts (HS68)and cervix carcinoma (HeLa)cells. Studies by confocal microscopy with specific markers of cell organelles, provided evidence that hypericin was accumulated in the Golgi apparatus. All these data shed a new light on in vitro drug delivery by using cell-released vesicles as carriers. (C) 2009 Elsevier B.V. All rights reserved.
SP - 206
EP - 215
DO - 10.1016/j.ijpharm.2009.06.039
UR - http://www.dx.doi.org/10.1016/j.ijpharm.2009.06.039
ER -