article · 17/06/2020
A simple device to immobilize protists for electrophysiology and microinjection
Résumé
We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol.
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Kulkarni, A., Elices, I., Escoubet, N., Pontani, L.-L., Prevost, A.-M., & Brette, R. (2020). A simple device to immobilize protists for electrophysiology and microinjection. Journal of Experimental Biology, 223(12), 1-5. https://doi.org/10.1242/jeb.219253@article{Kulkarni2020_387,
author = {Kulkarni, Anirudh and Elices, Irene and Escoubet, Nicolas and Pontani, Lea-Laetitia and Prevost, Alexis Michel and Brette, Romain},
year = {2020},
month = {6},
title = {A simple device to immobilize protists for electrophysiology and microinjection},
journal = {Journal of Experimental Biology},
volume = {223},
number = {12},
pages = {1-5},
abstract = {We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol.},
url = {https://journals.biologists.com/jeb/article/223/12/jeb219253/224563/A-simple-device-to-immobilize-protists-for},
doi = {10.1242/jeb.219253},
}TY - JOUR
AU - Kulkarni, Anirudh
AU - Elices, Irene
AU - Escoubet, Nicolas
AU - Pontani, Lea-Laetitia
AU - Prevost, Alexis Michel
AU - Brette, Romain
PY - 2020
DA - 2020/06/17
TI - A simple device to immobilize protists for electrophysiology and microinjection
JO - Journal of Experimental Biology
VL - 223
IS - 12
AB - We present a simple device to mechanically immobilize motile cells such as ciliates. It can be used in particular for intracellular electrophysiology and microinjection. A transparent filter with holes smaller than the specimen is stretched over an outlet. A flow is induced by either a peristaltic pump or a depressurized tank, mechanically entraining cells to the bottom, where they are immobilized against the filter. The cells start swimming again as soon as the flow is stopped. We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol.
SP - 1
EP - 5
DO - 10.1242/jeb.219253
UR - https://journals.biologists.com/jeb/article/223/12/jeb219253/224563/A-simple-device-to-immobilize-protists-for
ER -