article · 01/04/2023
A simple method to make, trap and deform a vesicle in a gel
Résumé
We present a simple method to produce giant lipid pseudo‑vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of α‑Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non‑intrusively, by indenting the surface of the gel.
Citer cet article
Tapie, P., Montel, L., Prevost, A., Pontani, L.-L., & Wandersman, E. (2023). A simple method to make, trap and deform a vesicle in a gel. Scientific Reports, 13(1), 5375. https://doi.org/https://doi.org/10.1038/s41598-023-31996-9@article{Tapie2023_382,
author = {Tapie, Pierre and Montel, Lorraine and Prevost, Alexis and Pontani, Léa-Laetitia and Wandersman, Elie},
year = {2023},
month = {4},
title = {A simple method to make, trap and deform a vesicle in a gel},
journal = {Scientific Reports},
volume = {13},
number = {1},
pages = {5375},
abstract = {We present a simple method to produce giant lipid pseudo‑vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of α‑Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non‑intrusively, by indenting the surface of the gel.},
url = {https://www.nature.com/articles/s41598-023-31996-9},
doi = {https://doi.org/10.1038/s41598-023-31996-9},
}TY - JOUR
AU - Tapie, Pierre
AU - Montel, Lorraine
AU - Prevost, Alexis
AU - Pontani, Léa-Laetitia
AU - Wandersman, Elie
PY - 2023
DA - 2023/04/01
TI - A simple method to make, trap and deform a vesicle in a gel
JO - Scientific Reports
VL - 13
IS - 1
AB - We present a simple method to produce giant lipid pseudo‑vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of α‑Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non‑intrusively, by indenting the surface of the gel.
SP - 5375
DO - https://doi.org/10.1038/s41598-023-31996-9
UR - https://www.nature.com/articles/s41598-023-31996-9
ER -