article · 01/09/2023
Random-access two-photon holographic optogenetic stimulation combined with brain-wide functional light-sheet imaging in larval zebrafish
Résumé
We describe a 3D holographic two-photon optogenetics setup based on acousto-optic deflectors combined with a custom-made compact light-sheet microscope setup. The system allows performing 3D-targeted perturbations of neuronal activity while simultaneously imaging the whole brain neuronal response over large field-of-view. This random-access holographic light patterning method offers high-speed sequential activation of dense neuronal assemblies spread over large areas while maintaining constant lateral and axial resolution. Here, we report on a systematic characterization of the stimulated neurons response and perform functional imaging on zebrafish (Danio Rerio) larvae while stimulating single neurons.
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Hubert, A., Dommanget-Kott, M., Wolf, S., Panier, T., Debrégeas, G., & Bormuth, V. (2023). Random-access two-photon holographic optogenetic stimulation combined with brain-wide functional light-sheet imaging in larval zebrafish. Advances in Microscopic Imaging IV, 12630, 11-14. https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12630/1263007/Random-access-two-photon-holographic-optogenetic-stimulation-combined-with-brain/10.1117/12.2671030.short?SSO=1@article{Hubert2023_380,
author = {Hubert, Antoine and Dommanget-Kott, Mattéo and Wolf, Sébastien and Panier, Thomas and Debrégeas, Georges and Bormuth, Volker},
year = {2023},
month = {9},
title = {Random-access two-photon holographic optogenetic stimulation combined with brain-wide functional light-sheet imaging in larval zebrafish},
journal = {Advances in Microscopic Imaging IV},
volume = {12630},
pages = {11-14},
abstract = {We describe a 3D holographic two-photon optogenetics setup based on acousto-optic deflectors combined with a custom-made compact light-sheet microscope setup. The system allows performing 3D-targeted perturbations of neuronal activity while simultaneously imaging the whole brain neuronal response over large field-of-view. This random-access holographic light patterning method offers high-speed sequential activation of dense neuronal assemblies spread over large areas while maintaining constant lateral and axial resolution. Here, we report on a systematic characterization of the stimulated neurons response and perform functional imaging on zebrafish (Danio Rerio) larvae while stimulating single neurons.},
url = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12630/1263007/Random-access-two-photon-holographic-optogenetic-stimulation-combined-with-brain/10.1117/12.2671030.short?SSO=1},
}TY - JOUR
AU - Hubert, Antoine
AU - Dommanget-Kott, Mattéo
AU - Wolf, Sébastien
AU - Panier, Thomas
AU - Debrégeas, Georges
AU - Bormuth, Volker
PY - 2023
DA - 2023/09/01
TI - Random-access two-photon holographic optogenetic stimulation combined with brain-wide functional light-sheet imaging in larval zebrafish
JO - Advances in Microscopic Imaging IV
VL - 12630
AB - We describe a 3D holographic two-photon optogenetics setup based on acousto-optic deflectors combined with a custom-made compact light-sheet microscope setup. The system allows performing 3D-targeted perturbations of neuronal activity while simultaneously imaging the whole brain neuronal response over large field-of-view. This random-access holographic light patterning method offers high-speed sequential activation of dense neuronal assemblies spread over large areas while maintaining constant lateral and axial resolution. Here, we report on a systematic characterization of the stimulated neurons response and perform functional imaging on zebrafish (Danio Rerio) larvae while stimulating single neurons.
SP - 11
EP - 14
UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12630/1263007/Random-access-two-photon-holographic-optogenetic-stimulation-combined-with-brain/10.1117/12.2671030.short?SSO=1
ER -