article · 28/09/2023
Magnetic actuation of otoliths allows behavioral and brain-wide neuronal exploration of vestibulo-motor processing in larval zebrafish
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Résumé
The vestibular system in the inner ear plays a central role in sensorimotor control by informing the brain about the orientation and acceleration of the head. However, most experiments in neurophysiology are performed using head-fixed configurations, depriving animals of vestibular inputs. To overcome this limitation, we decorated the utricular otolith of the vestibular system in larval zebrafish with paramagnetic nanoparticles. This procedure effectively endowed the animal with magneto-sensitive capacities: applied magnetic field gradients induced forces on the otoliths, resulting in robust behavioral responses comparable to those evoked by rotating the animal by up to 25°. We recorded the whole-brain neuronal response to this fictive motion stimulation using light-sheet functional imaging. Experiments performed in unilaterally injected fish revealed the activation of a commissural inhibition between the brain hemispheres. This magnetic-based stimulation technique for larval zebrafish opens new perspectives to functionally dissect the neural circuits underlying vestibular processing and to develop multisensory virtual environments, including vestibular feedback.
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Beiza-Canelo, N., Moulle, H., Pujol, T., Panier, T., Migault, G., Le Goc, G., Tapie, P., Desprat, N., Straka, H., Debrégeas, G., & Bormuth, V. (2023). Magnetic actuation of otoliths allows behavioral and brain-wide neuronal exploration of vestibulo-motor processing in larval zebrafish. Current Biology, 33(12), 2438-2448.e6. https://doi.org/https://doi.org/10.1016/j.cub.2023.05.026@article{BeizaCanelo2023_379,
author = {Beiza-Canelo, Natalia and Moulle, Hippolyte and Pujol, Thomas and Panier, Thomas and Migault, Geoffrey and Le Goc, Guillaume and Tapie, Pierre and Desprat, Nicolas and Straka, Hans and Debrégeas, Georges and Bormuth, Volker},
year = {2023},
month = {9},
title = {Magnetic actuation of otoliths allows behavioral and brain-wide neuronal exploration of vestibulo-motor processing in larval zebrafish},
journal = {Current Biology},
volume = {33},
number = {12},
pages = {2438-2448.e6},
abstract = {The vestibular system in the inner ear plays a central role in sensorimotor control by informing the brain about the orientation and acceleration of the head. However, most experiments in neurophysiology are performed using head-fixed configurations, depriving animals of vestibular inputs. To overcome this limitation, we decorated the utricular otolith of the vestibular system in larval zebrafish with paramagnetic nanoparticles. This procedure effectively endowed the animal with magneto-sensitive capacities: applied magnetic field gradients induced forces on the otoliths, resulting in robust behavioral responses comparable to those evoked by rotating the animal by up to 25°. We recorded the whole-brain neuronal response to this fictive motion stimulation using light-sheet functional imaging. Experiments performed in unilaterally injected fish revealed the activation of a commissural inhibition between the brain hemispheres. This magnetic-based stimulation technique for larval zebrafish opens new perspectives to functionally dissect the neural circuits underlying vestibular processing and to develop multisensory virtual environments, including vestibular feedback.},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0960982223006218},
doi = {https://doi.org/10.1016/j.cub.2023.05.026},
}TY - JOUR
AU - Beiza-Canelo, Natalia
AU - Moulle, Hippolyte
AU - Pujol, Thomas
AU - Panier, Thomas
AU - Migault, Geoffrey
AU - Le Goc, Guillaume
AU - Tapie, Pierre
AU - Desprat, Nicolas
AU - Straka, Hans
AU - Debrégeas, Georges
AU - Bormuth, Volker
PY - 2023
DA - 2023/09/28
TI - Magnetic actuation of otoliths allows behavioral and brain-wide neuronal exploration of vestibulo-motor processing in larval zebrafish
JO - Current Biology
VL - 33
IS - 12
AB - The vestibular system in the inner ear plays a central role in sensorimotor control by informing the brain about the orientation and acceleration of the head. However, most experiments in neurophysiology are performed using head-fixed configurations, depriving animals of vestibular inputs. To overcome this limitation, we decorated the utricular otolith of the vestibular system in larval zebrafish with paramagnetic nanoparticles. This procedure effectively endowed the animal with magneto-sensitive capacities: applied magnetic field gradients induced forces on the otoliths, resulting in robust behavioral responses comparable to those evoked by rotating the animal by up to 25°. We recorded the whole-brain neuronal response to this fictive motion stimulation using light-sheet functional imaging. Experiments performed in unilaterally injected fish revealed the activation of a commissural inhibition between the brain hemispheres. This magnetic-based stimulation technique for larval zebrafish opens new perspectives to functionally dissect the neural circuits underlying vestibular processing and to develop multisensory virtual environments, including vestibular feedback.
SP - 2438
EP - 2448.e6
DO - https://doi.org/10.1016/j.cub.2023.05.026
UR - https://www.sciencedirect.com/science/article/abs/pii/S0960982223006218
ER -