article · 11/07/2023
A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design
Résumé
Two-photon light sheet microscopy offers great potential for a range of biological applications, but its practical implementation is impeded by the high cost of laser sources, the complexity of construction, and the challenges associated with adapting to existing microscope setups. Here, we release an open-source design that addresses these limitations by providing detailed building instructions for the transformation of a brightfield microscope into a versatile one- and two-photon light sheet system. Our design incorporates a specially designed broadband hollow core fiber, enabling the simultaneous utilization of an expansive pulsed laser source from another setup alongside a visible laser. This integration allows for uncompromised image resolution and speed. Furthermore, the design reduces the complexity of construction, alignment, and overall cost, thereby significantly enhancing the accessibility of this technology (https://github.com/LJPZebra/OLU).Competing Interest StatementThe authors have declared no competing interest.
Citer cet article
Panier, T., Migault, G., Hubert, A., Trentesaux, H., Beaudou, B., Debrégeas, G., & Bormuth, V. (2023). A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design. BioRxiv. https://doi.org/https://doi.org/10.1101/2023.07.10.548107@article{Panier2023_378,
author = {Panier, Thomas and Migault, Geoffrey and Hubert, Antoine and Trentesaux, Hugo and Beaudou, Benoît and Debrégeas, Georges and Bormuth, Volker},
year = {2023},
month = {7},
title = {A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design},
journal = {BioRxiv},
abstract = {Two-photon light sheet microscopy offers great potential for a range of biological applications, but its practical implementation is impeded by the high cost of laser sources, the complexity of construction, and the challenges associated with adapting to existing microscope setups. Here, we release an open-source design that addresses these limitations by providing detailed building instructions for the transformation of a brightfield microscope into a versatile one- and two-photon light sheet system. Our design incorporates a specially designed broadband hollow core fiber, enabling the simultaneous utilization of an expansive pulsed laser source from another setup alongside a visible laser. This integration allows for uncompromised image resolution and speed. Furthermore, the design reduces the complexity of construction, alignment, and overall cost, thereby significantly enhancing the accessibility of this technology (https://github.com/LJPZebra/OLU).Competing Interest StatementThe authors have declared no competing interest.},
doi = {https://doi.org/10.1101/2023.07.10.548107},
}TY - JOUR
AU - Panier, Thomas
AU - Migault, Geoffrey
AU - Hubert, Antoine
AU - Trentesaux, Hugo
AU - Beaudou, Benoît
AU - Debrégeas, Georges
AU - Bormuth, Volker
PY - 2023
DA - 2023/07/11
TI - A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design
JO - BioRxiv
AB - Two-photon light sheet microscopy offers great potential for a range of biological applications, but its practical implementation is impeded by the high cost of laser sources, the complexity of construction, and the challenges associated with adapting to existing microscope setups. Here, we release an open-source design that addresses these limitations by providing detailed building instructions for the transformation of a brightfield microscope into a versatile one- and two-photon light sheet system. Our design incorporates a specially designed broadband hollow core fiber, enabling the simultaneous utilization of an expansive pulsed laser source from another setup alongside a visible laser. This integration allows for uncompromised image resolution and speed. Furthermore, the design reduces the complexity of construction, alignment, and overall cost, thereby significantly enhancing the accessibility of this technology (https://github.com/LJPZebra/OLU).Competing Interest StatementThe authors have declared no competing interest.
DO - https://doi.org/10.1101/2023.07.10.548107
ER -