proceeding · 01/01/2011
Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre
Résumé
We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.
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Peyrot, D.-A., Lefort, C., Haidar, D.-A., Mansuryan, T., Kruglik, S., Sandeau, N., Louradour, F., & Bourg-Heckly, G. (2011). Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre. ENDOSCOPIC MICROSCOPY VI, 7893(789309). https://doi.org/10.1117/12.875440@inproceedings{Peyrot2011_35,
author = {Peyrot, Donald A. and Lefort, Claire and Haidar, Darine Abi and Mansuryan, Tigran and Kruglik, Sergei and Sandeau, Nicolas and Louradour, Frederic and Bourg-Heckly, Genevieve},
year = {2011},
month = {1},
title = {Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre},
booktitle = {ENDOSCOPIC MICROSCOPY VI},
publisher = {SPIE-INT SOC OPTICAL ENGINEERING},
volume = {7893},
number = {789309},
series = {Proceedings of SPIE},
address = {1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA},
abstract = {We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.},
note = {Conference on Endoscopic Microscopy VI, San Francisco, CA, JAN 23-24, 2011},
url = {http://www.dx.doi.org/10.1117/12.875440},
doi = {10.1117/12.875440},
issn = {0277-786X},
}TY - CONF
AU - Peyrot, Donald A.
AU - Lefort, Claire
AU - Haidar, Darine Abi
AU - Mansuryan, Tigran
AU - Kruglik, Sergei
AU - Sandeau, Nicolas
AU - Louradour, Frederic
AU - Bourg-Heckly, Genevieve
PY - 2011
DA - 2011/01/01
TI - Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre
BT - ENDOSCOPIC MICROSCOPY VI
VL - 7893
IS - 789309
PB - SPIE-INT SOC OPTICAL ENGINEERING
SN - 0277-786X
AB - We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.
DO - 10.1117/12.875440
UR - http://www.dx.doi.org/10.1117/12.875440
ER -