article · 01/03/2011
Sub-picosecond Raman spectrometer for time-resolved studies of structural dynamics in heme proteins
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Résumé
We describe a pump-probe Raman spectrometer based on a femtosecond Ti:sapphire laser, an optical parametric generator and two optical parametric amplifiers for time-resolved studies, with emphasis on the structural dynamics in heme proteins. The system provides a 100-fs pump pulse tunable in the range 500-600 nm and a transform-limited sub-picosecond probe pulse tunable in the range 390-450 nm. The spectrometer has spectral (25 cm(-1)) and temporal (similar to 0.7 ps) resolutions which constitute an effective compromise for identifying transient heme protein species and for following their structural evolution by spontaneous Raman scattering in the time range 0.5 ps to 2 ns. This apparatus was applied to time-resolved studies of a broad range of heme proteins, monitoring the primary dynamics of photoinduced heme coordination state and structural changes, its interaction with protein side-chains and diatomic gaseous ligands, as well as heme vibrational cooling. The treatment of transient Raman spectra is described in detail, and the advantages and shortcomings of spontaneous resonance Raman spectroscopy for ultrafast heme proteins studies are discussed. We demonstrate the efficiency of the constructed spectrometer by measuring Raman spectra in the sub-picosecond and picosecond time ranges for the oxygen-storage heme protein myoglobin and for the oxygen-sensor heme protein FixLH in interaction with the diatomic gaseous ligands CO, NO, and O-2. Copyright (C) 2010 John Wiley \& Sons, Ltd.
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Kruglik, S.-G., Lambry, J.-C., Martin, J.-L., Vos, M.-H., & Negrerie, M. (2011). Sub-picosecond Raman spectrometer for time-resolved studies of structural dynamics in heme proteins. Journal of Raman Spectroscopy, 42(3), 265-275. https://doi.org/10.1002/jrs.2685@article{Kruglik2011_34,
author = {Kruglik, Sergei G. and Lambry, Jean-Christophe and Martin, Jean-Louis and Vos, Marten H. and Negrerie, Michel},
year = {2011},
month = {3},
title = {Sub-picosecond Raman spectrometer for time-resolved studies of structural dynamics in heme proteins},
journal = {Journal of Raman Spectroscopy},
publisher = {WILEY-BLACKWELL},
volume = {42},
number = {3},
pages = {265-275},
address = {COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA},
abstract = {We describe a pump-probe Raman spectrometer based on a femtosecond Ti:sapphire laser, an optical parametric generator and two optical parametric amplifiers for time-resolved studies, with emphasis on the structural dynamics in heme proteins. The system provides a 100-fs pump pulse tunable in the range 500-600 nm and a transform-limited sub-picosecond probe pulse tunable in the range 390-450 nm. The spectrometer has spectral (25 cm(-1)) and temporal (similar to 0.7 ps) resolutions which constitute an effective compromise for identifying transient heme protein species and for following their structural evolution by spontaneous Raman scattering in the time range 0.5 ps to 2 ns. This apparatus was applied to time-resolved studies of a broad range of heme proteins, monitoring the primary dynamics of photoinduced heme coordination state and structural changes, its interaction with protein side-chains and diatomic gaseous ligands, as well as heme vibrational cooling. The treatment of transient Raman spectra is described in detail, and the advantages and shortcomings of spontaneous resonance Raman spectroscopy for ultrafast heme proteins studies are discussed. We demonstrate the efficiency of the constructed spectrometer by measuring Raman spectra in the sub-picosecond and picosecond time ranges for the oxygen-storage heme protein myoglobin and for the oxygen-sensor heme protein FixLH in interaction with the diatomic gaseous ligands CO, NO, and O-2. Copyright (C) 2010 John Wiley \textbackslash{}\& Sons, Ltd.},
url = {http://www.dx.doi.org/10.1002/jrs.2685},
doi = {10.1002/jrs.2685},
issn = {0377-0486},
}TY - JOUR
AU - Kruglik, Sergei G.
AU - Lambry, Jean-Christophe
AU - Martin, Jean-Louis
AU - Vos, Marten H.
AU - Negrerie, Michel
PY - 2011
DA - 2011/03/01
TI - Sub-picosecond Raman spectrometer for time-resolved studies of structural dynamics in heme proteins
JO - Journal of Raman Spectroscopy
VL - 42
IS - 3
PB - WILEY-BLACKWELL
SN - 0377-0486
AB - We describe a pump-probe Raman spectrometer based on a femtosecond Ti:sapphire laser, an optical parametric generator and two optical parametric amplifiers for time-resolved studies, with emphasis on the structural dynamics in heme proteins. The system provides a 100-fs pump pulse tunable in the range 500-600 nm and a transform-limited sub-picosecond probe pulse tunable in the range 390-450 nm. The spectrometer has spectral (25 cm(-1)) and temporal (similar to 0.7 ps) resolutions which constitute an effective compromise for identifying transient heme protein species and for following their structural evolution by spontaneous Raman scattering in the time range 0.5 ps to 2 ns. This apparatus was applied to time-resolved studies of a broad range of heme proteins, monitoring the primary dynamics of photoinduced heme coordination state and structural changes, its interaction with protein side-chains and diatomic gaseous ligands, as well as heme vibrational cooling. The treatment of transient Raman spectra is described in detail, and the advantages and shortcomings of spontaneous resonance Raman spectroscopy for ultrafast heme proteins studies are discussed. We demonstrate the efficiency of the constructed spectrometer by measuring Raman spectra in the sub-picosecond and picosecond time ranges for the oxygen-storage heme protein myoglobin and for the oxygen-sensor heme protein FixLH in interaction with the diatomic gaseous ligands CO, NO, and O-2. Copyright (C) 2010 John Wiley \& Sons, Ltd.
SP - 265
EP - 275
DO - 10.1002/jrs.2685
UR - http://www.dx.doi.org/10.1002/jrs.2685
ER -