article · 23/10/2019
Characterization of a multicore fiber image guide for nonlinear endoscopic imaging using two-photon fluorescence and second-harmonic generation
Résumé
Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MPM to clinical endoscopy. We present real-time SHG and 2PEF ex vivo images using an endomicroscope, which totally complies with clinical endoscopy regulations. This system is based on the proximal scanning of a commercial multicore image guide (IG). For understanding the inhomogeneities of the recorded images, we quantitatively characterize the IG at the single-core level during nonlinear excitation. The obtained results suggest that these inhomogeneities originate from the variable core geometries that, therefore, exhibit variable nonlinear and dispersive properties. Finally, we propose a method based on modulation of dispersion precompensation to address the image inhomogeneity issue and, as a proof of concept, we demonstrate its capability to improve the nonlinear image quality.
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Garofalakis, A., Kruglik, S.-G., Mansuryan, T., Gillibert, A., Thiberville, L., Louradour, F., Vever-Bizet, C., & Bourg-Heckly, G. (2019). Characterization of a multicore fiber image guide for nonlinear endoscopic imaging using two-photon fluorescence and second-harmonic generation. Journal of Biomedical Optics, 24(10), article N° 106004. https://doi.org/10.1117/1.JBO.24.10.106004@article{Garofalakis2019_313,
author = {Garofalakis, A. and Kruglik, S. G. and Mansuryan, T. and Gillibert, A. and Thiberville, L. and Louradour, F. and Vever-Bizet, C. and Bourg-Heckly, G.},
year = {2019},
month = {10},
title = {Characterization of a multicore fiber image guide for nonlinear endoscopic imaging using two-photon fluorescence and second-harmonic generation},
journal = {Journal of Biomedical Optics},
volume = {24},
number = {10},
pages = {article N° 106004},
abstract = {Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MPM to clinical endoscopy. We present real-time SHG and 2PEF ex vivo images using an endomicroscope, which totally complies with clinical endoscopy regulations. This system is based on the proximal scanning of a commercial multicore image guide (IG). For understanding the inhomogeneities of the recorded images, we quantitatively characterize the IG at the single-core level during nonlinear excitation. The obtained results suggest that these inhomogeneities originate from the variable core geometries that, therefore, exhibit variable nonlinear and dispersive properties. Finally, we propose a method based on modulation of dispersion precompensation to address the image inhomogeneity issue and, as a proof of concept, we demonstrate its capability to improve the nonlinear image quality.},
url = {https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-24/issue-10/106004/Characterization-of-a-multicore-fiber-image-guide-for-nonlinear-endoscopic/10.1117/1.JBO.24.10.106004.full?SSO=1},
doi = {10.1117/1.JBO.24.10.106004},
}TY - JOUR
AU - Garofalakis, A.
AU - Kruglik, S. G.
AU - Mansuryan, T.
AU - Gillibert, A.
AU - Thiberville, L.
AU - Louradour, F.
AU - Vever-Bizet, C.
AU - Bourg-Heckly, G.
PY - 2019
DA - 2019/10/23
TI - Characterization of a multicore fiber image guide for nonlinear endoscopic imaging using two-photon fluorescence and second-harmonic generation
JO - Journal of Biomedical Optics
VL - 24
IS - 10
AB - Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MPM to clinical endoscopy. We present real-time SHG and 2PEF ex vivo images using an endomicroscope, which totally complies with clinical endoscopy regulations. This system is based on the proximal scanning of a commercial multicore image guide (IG). For understanding the inhomogeneities of the recorded images, we quantitatively characterize the IG at the single-core level during nonlinear excitation. The obtained results suggest that these inhomogeneities originate from the variable core geometries that, therefore, exhibit variable nonlinear and dispersive properties. Finally, we propose a method based on modulation of dispersion precompensation to address the image inhomogeneity issue and, as a proof of concept, we demonstrate its capability to improve the nonlinear image quality.
SP - article N° 106004
DO - 10.1117/1.JBO.24.10.106004
UR - https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-24/issue-10/106004/Characterization-of-a-multicore-fiber-image-guide-for-nonlinear-endoscopic/10.1117/1.JBO.24.10.106004.full?SSO=1
ER -