article · 18/02/2018
Archaeal cells share common size control with bacteria despite noisier growth and division
Résumé
In nature, microorganisms exhibit different volumes spanning six orders of magnitude 1 . Despite their capability to create different sizes, a clonal population in a given environment maintains a uniform size across individual cells. Recent studies in eukaryotic and bacterial organisms showed that this homogeneity in cell size can be accomplished by growing a constant size between two cell cycle events (that is, the adder model 2-6 ). Demonstration of the adder model led to the hypothesis that this phenomenon is a consequence of convergent evolution. Given that archaeal cells share characteristics with both bacteria and eukaryotes, we investigated whether and how archaeal cells exhibit control over cell size. To this end, we developed a soft-lithography method of growing the archaeal cells to enable quantitative time-lapse imaging and single-cell analysis, which would be useful for other microorganisms. Using this method, we demonstrated that Halobacterium?salinarum, a hypersaline-adapted archaeal organism, grows exponentially at the single-cell level and maintains a narrow-size distribution by adding a constant length between cell division events. Interestingly, the archaeal cells exhibited greater variability in cell division placement and exponential growth rate across individual cells in a population relative to those observed in Escherichia?coli 6-9 . Here, we present a theoretical framework that explains how these larger fluctuations in archaeal cell cycle events contribute to cell size variability and control.
Citer cet article
Eun, Y.-J., Ho, P.-Y., Kim, M., LaRussa, S., Robert, L., Renner, L., Schmid, A., Garner, E., & Amir, A. (2018). Archaeal cells share common size control with bacteria despite noisier growth and division. Nature Microbiology, 3, 148-154. https://doi.org/10.1038/s41564-017-0082-6@article{Eun2018_304,
author = {Eun, Ye-Jin and Ho, Po-Yi and Kim, Minjeong and LaRussa, Salvatore and Robert, Lydia and Renner, Lars and Schmid, Amy and Garner, Ethan and Amir, Ariel},
year = {2018},
month = {2},
title = {Archaeal cells share common size control with bacteria despite noisier growth and division},
journal = {Nature Microbiology},
volume = {3},
pages = {148-154},
abstract = {In nature, microorganisms exhibit different volumes spanning six orders of magnitude 1 . Despite their capability to create different sizes, a clonal population in a given environment maintains a uniform size across individual cells. Recent studies in eukaryotic and bacterial organisms showed that this homogeneity in cell size can be accomplished by growing a constant size between two cell cycle events (that is, the adder model 2-6 ). Demonstration of the adder model led to the hypothesis that this phenomenon is a consequence of convergent evolution. Given that archaeal cells share characteristics with both bacteria and eukaryotes, we investigated whether and how archaeal cells exhibit control over cell size. To this end, we developed a soft-lithography method of growing the archaeal cells to enable quantitative time-lapse imaging and single-cell analysis, which would be useful for other microorganisms. Using this method, we demonstrated that Halobacterium?salinarum, a hypersaline-adapted archaeal organism, grows exponentially at the single-cell level and maintains a narrow-size distribution by adding a constant length between cell division events. Interestingly, the archaeal cells exhibited greater variability in cell division placement and exponential growth rate across individual cells in a population relative to those observed in Escherichia?coli 6-9 . Here, we present a theoretical framework that explains how these larger fluctuations in archaeal cell cycle events contribute to cell size variability and control.},
doi = {10.1038/s41564-017-0082-6},
}TY - JOUR
AU - Eun, Ye-Jin
AU - Ho, Po-Yi
AU - Kim, Minjeong
AU - LaRussa, Salvatore
AU - Robert, Lydia
AU - Renner, Lars
AU - Schmid, Amy
AU - Garner, Ethan
AU - Amir, Ariel
PY - 2018
DA - 2018/02/18
TI - Archaeal cells share common size control with bacteria despite noisier growth and division
JO - Nature Microbiology
VL - 3
AB - In nature, microorganisms exhibit different volumes spanning six orders of magnitude 1 . Despite their capability to create different sizes, a clonal population in a given environment maintains a uniform size across individual cells. Recent studies in eukaryotic and bacterial organisms showed that this homogeneity in cell size can be accomplished by growing a constant size between two cell cycle events (that is, the adder model 2-6 ). Demonstration of the adder model led to the hypothesis that this phenomenon is a consequence of convergent evolution. Given that archaeal cells share characteristics with both bacteria and eukaryotes, we investigated whether and how archaeal cells exhibit control over cell size. To this end, we developed a soft-lithography method of growing the archaeal cells to enable quantitative time-lapse imaging and single-cell analysis, which would be useful for other microorganisms. Using this method, we demonstrated that Halobacterium?salinarum, a hypersaline-adapted archaeal organism, grows exponentially at the single-cell level and maintains a narrow-size distribution by adding a constant length between cell division events. Interestingly, the archaeal cells exhibited greater variability in cell division placement and exponential growth rate across individual cells in a population relative to those observed in Escherichia?coli 6-9 . Here, we present a theoretical framework that explains how these larger fluctuations in archaeal cell cycle events contribute to cell size variability and control.
SP - 148
EP - 154
DO - 10.1038/s41564-017-0082-6
ER -