article · 01/05/2012
Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration
Résumé
Several major lung pathologies are characterized by early modifications of the extracellular matrix (ECM) fibrillar collagen and elastin network. We report here the development of a nonlinear fiber-optic spectrometer, compatible with an endoscopic use, primarily intended for the recording of second-harmonic generation (SHG) signal of collagen and two-photon excited fluorescence (2PEF) of both collagen and elastin. Fiber dispersion is accurately compensated by the use of a specific grism-pair stretcher, allowing laser pulse temporal width around 70 fs and excitation wavelength tunability from 790 to 900 nm. This spectrometer was used to investigate the excitation wavelength dependence (from 800 to 870 nm) of SHG and 2PEF spectra originating from ex vivo human lung tissue samples. The results were compared with spectral responses of collagen gel and elastin powder reference samples and also with data obtained using standard nonlinear microspectroscopy. The excitation-wavelength-tunable nonlinear fiber-optic spectrometer presented in this study allows performing nonlinear spectroscopy of human lung tissue ECM through the elastin 2PEF and the collagen SHG signals. This work opens the way to tunable excitation nonlinear endomicroscopy based on both distal scanning of a single optical fiber and proximal scanning of a fiber-optic bundle. (c) 2012 Optical Society of America
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Peyrot, D.-A., Lefort, C., Steffenhagen, M., Mansuryan, T., Ducourthial, G., Abi-Haidar, D., Sandeau, N., Vever-Bizet, C., Kruglik, S.-G., Thiberville, L., Louradour, F., & Bourg-Heckly, G. (2012). Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration. Biomedical Optics Express, 3(5), 840-853. http://www.dx.doi.org/@article{Peyrot2012_30,
author = {Peyrot, Donald A. and Lefort, Claire and Steffenhagen, Marie and Mansuryan, Tigran and Ducourthial, Guillaume and Abi-Haidar, Darine and Sandeau, Nicolas and Vever-Bizet, Christine and Kruglik, Sergei G. and Thiberville, Luc and Louradour, Frederic and Bourg-Heckly, Genevieve},
year = {2012},
month = {5},
title = {Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration},
journal = {Biomedical Optics Express},
publisher = {OPTICAL SOC AMER},
volume = {3},
number = {5},
pages = {840-853},
address = {2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA},
abstract = {Several major lung pathologies are characterized by early modifications of the extracellular matrix (ECM) fibrillar collagen and elastin network. We report here the development of a nonlinear fiber-optic spectrometer, compatible with an endoscopic use, primarily intended for the recording of second-harmonic generation (SHG) signal of collagen and two-photon excited fluorescence (2PEF) of both collagen and elastin. Fiber dispersion is accurately compensated by the use of a specific grism-pair stretcher, allowing laser pulse temporal width around 70 fs and excitation wavelength tunability from 790 to 900 nm. This spectrometer was used to investigate the excitation wavelength dependence (from 800 to 870 nm) of SHG and 2PEF spectra originating from ex vivo human lung tissue samples. The results were compared with spectral responses of collagen gel and elastin powder reference samples and also with data obtained using standard nonlinear microspectroscopy. The excitation-wavelength-tunable nonlinear fiber-optic spectrometer presented in this study allows performing nonlinear spectroscopy of human lung tissue ECM through the elastin 2PEF and the collagen SHG signals. This work opens the way to tunable excitation nonlinear endomicroscopy based on both distal scanning of a single optical fiber and proximal scanning of a fiber-optic bundle. (c) 2012 Optical Society of America},
url = {http://www.dx.doi.org/},
issn = {2156-7085},
}TY - JOUR
AU - Peyrot, Donald A.
AU - Lefort, Claire
AU - Steffenhagen, Marie
AU - Mansuryan, Tigran
AU - Ducourthial, Guillaume
AU - Abi-Haidar, Darine
AU - Sandeau, Nicolas
AU - Vever-Bizet, Christine
AU - Kruglik, Sergei G.
AU - Thiberville, Luc
AU - Louradour, Frederic
AU - Bourg-Heckly, Genevieve
PY - 2012
DA - 2012/05/01
TI - Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration
JO - Biomedical Optics Express
VL - 3
IS - 5
PB - OPTICAL SOC AMER
SN - 2156-7085
AB - Several major lung pathologies are characterized by early modifications of the extracellular matrix (ECM) fibrillar collagen and elastin network. We report here the development of a nonlinear fiber-optic spectrometer, compatible with an endoscopic use, primarily intended for the recording of second-harmonic generation (SHG) signal of collagen and two-photon excited fluorescence (2PEF) of both collagen and elastin. Fiber dispersion is accurately compensated by the use of a specific grism-pair stretcher, allowing laser pulse temporal width around 70 fs and excitation wavelength tunability from 790 to 900 nm. This spectrometer was used to investigate the excitation wavelength dependence (from 800 to 870 nm) of SHG and 2PEF spectra originating from ex vivo human lung tissue samples. The results were compared with spectral responses of collagen gel and elastin powder reference samples and also with data obtained using standard nonlinear microspectroscopy. The excitation-wavelength-tunable nonlinear fiber-optic spectrometer presented in this study allows performing nonlinear spectroscopy of human lung tissue ECM through the elastin 2PEF and the collagen SHG signals. This work opens the way to tunable excitation nonlinear endomicroscopy based on both distal scanning of a single optical fiber and proximal scanning of a fiber-optic bundle. (c) 2012 Optical Society of America
SP - 840
EP - 853
UR - http://www.dx.doi.org/
ER -