article · 17/03/2017
Inflammation boosts bacteriophage transfer between Salmonella spp.
Résumé
Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. Amurine Salmonella Typhimurium (S.Tm) diarrhea model was used to study the transfer of SopEF, a prophage from S.Tm SL1344, to S.Tm ATCC14028S. Gut inflammation and enteric disease triggered >55\% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEF transfer was reduced by up to 10(5)-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal S.Tm population from inducing inflammation and consequently abolished SopEF transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.
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Diard, M., Bakkeren, E., Cornuault, J.-K., Moor, K., Hausmann, A., Sellin, M.-E., Loverdo, C., Aertsen, A., Ackermann, M., De Paepe, M., Slack, E., & Hardt, W.-D. (2017). Inflammation boosts bacteriophage transfer between Salmonella spp. Science, 355(6330, SI). https://doi.org/10.1126/science.aaf8451@article{Diard2017_296,
author = {Diard, Mederic and Bakkeren, Erik and Cornuault, Jeffrey K. and Moor, Kathrin and Hausmann, Annika and Sellin, Mikael E. and Loverdo, Claude and Aertsen, Abram and Ackermann, Martin and De Paepe, Marianne and Slack, Emma and Hardt, Wolf-Dietrich},
year = {2017},
month = {3},
title = {Inflammation boosts bacteriophage transfer between Salmonella spp.},
journal = {Science},
publisher = {AMER ASSOC ADVANCEMENT SCIENCE},
volume = {355},
number = {6330, SI},
address = {1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA},
abstract = {Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. Amurine Salmonella Typhimurium (S.Tm) diarrhea model was used to study the transfer of SopEF, a prophage from S.Tm SL1344, to S.Tm ATCC14028S. Gut inflammation and enteric disease triggered >55\textbackslash{}\% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEF transfer was reduced by up to 10(5)-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal S.Tm population from inducing inflammation and consequently abolished SopEF transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.},
url = {http://www.dx.doi.org/10.1126/science.aaf8451},
doi = {10.1126/science.aaf8451},
issn = {0036-8075},
}TY - JOUR
AU - Diard, Mederic
AU - Bakkeren, Erik
AU - Cornuault, Jeffrey K.
AU - Moor, Kathrin
AU - Hausmann, Annika
AU - Sellin, Mikael E.
AU - Loverdo, Claude
AU - Aertsen, Abram
AU - Ackermann, Martin
AU - De Paepe, Marianne
AU - Slack, Emma
AU - Hardt, Wolf-Dietrich
PY - 2017
DA - 2017/03/17
TI - Inflammation boosts bacteriophage transfer between Salmonella spp.
JO - Science
VL - 355
IS - 6330, SI
PB - AMER ASSOC ADVANCEMENT SCIENCE
SN - 0036-8075
AB - Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. Amurine Salmonella Typhimurium (S.Tm) diarrhea model was used to study the transfer of SopEF, a prophage from S.Tm SL1344, to S.Tm ATCC14028S. Gut inflammation and enteric disease triggered >55\% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEF transfer was reduced by up to 10(5)-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal S.Tm population from inducing inflammation and consequently abolished SopEF transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.
DO - 10.1126/science.aaf8451
UR - http://www.dx.doi.org/10.1126/science.aaf8451
ER -