article · 28/06/2018
Intermittent Pili-Mediated Forces Fluidize Neisseria meningitidis Aggregates Promoting Vascular Colonization
Résumé
Neisseria meningitidis, a bacterium responsible for meningitis and septicemia, proliferates and eventually fills the lumen of blood capillaries with multicellular aggregates. The impact of this aggregation process and its specific properties are unknown. We first show that aggregative properties are necessary for efficient infection and study their underlying physical mechanisms. Micropipette aspiration and single-cell tracking unravel unique features of an atypical fluidized phase, with single-cell diffusion exceeding that of isolated cells. A quantitative description of the bacterial pair interactions combined with active matter physics-based modeling show that this behavior relies on type IV pili active dynamics that mediate alternating phases of bacteria fast mutual approach, contact, and release. These peculiar fluid properties proved necessary to adjust to the geometry of capillaries upon bacterial proliferation. Intermittent attractive forces thus generate a fluidized phase that allows for efficient colonization of the blood capillary network during infection.
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Bonazzi, D., Lo Schiavo, V., Machata, S., Djafer-Cherif, I., Nivoit, P., Manriquez, V., Tanimoto, H., Husson, J., Henry, N., Chate, H., Voituriez, R., & Dumenil, G. (2018). Intermittent Pili-Mediated Forces Fluidize Neisseria meningitidis Aggregates Promoting Vascular Colonization. Cell, 174(1). https://doi.org/10.1016/j.cell.2018.04.010@article{Bonazzi2018_282,
author = {Bonazzi, Daria and Lo Schiavo, Valentina and Machata, Silke and Djafer-Cherif, Ilyas and Nivoit, Pierre and Manriquez, Valeria and Tanimoto, Hirokazu and Husson, Julien and Henry, Nelly and Chate, Hugues and Voituriez, Raphael and Dumenil, Guillaume},
year = {2018},
month = {6},
title = {Intermittent Pili-Mediated Forces Fluidize Neisseria meningitidis Aggregates Promoting Vascular Colonization},
journal = {Cell},
publisher = {CELL PRESS},
volume = {174},
number = {1},
address = {50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA},
abstract = {Neisseria meningitidis, a bacterium responsible for meningitis and septicemia, proliferates and eventually fills the lumen of blood capillaries with multicellular aggregates. The impact of this aggregation process and its specific properties are unknown. We first show that aggregative properties are necessary for efficient infection and study their underlying physical mechanisms. Micropipette aspiration and single-cell tracking unravel unique features of an atypical fluidized phase, with single-cell diffusion exceeding that of isolated cells. A quantitative description of the bacterial pair interactions combined with active matter physics-based modeling show that this behavior relies on type IV pili active dynamics that mediate alternating phases of bacteria fast mutual approach, contact, and release. These peculiar fluid properties proved necessary to adjust to the geometry of capillaries upon bacterial proliferation. Intermittent attractive forces thus generate a fluidized phase that allows for efficient colonization of the blood capillary network during infection.},
url = {http://www.dx.doi.org/10.1016/j.cell.2018.04.010},
doi = {10.1016/j.cell.2018.04.010},
issn = {0092-8674},
}TY - JOUR
AU - Bonazzi, Daria
AU - Lo Schiavo, Valentina
AU - Machata, Silke
AU - Djafer-Cherif, Ilyas
AU - Nivoit, Pierre
AU - Manriquez, Valeria
AU - Tanimoto, Hirokazu
AU - Husson, Julien
AU - Henry, Nelly
AU - Chate, Hugues
AU - Voituriez, Raphael
AU - Dumenil, Guillaume
PY - 2018
DA - 2018/06/28
TI - Intermittent Pili-Mediated Forces Fluidize Neisseria meningitidis Aggregates Promoting Vascular Colonization
JO - Cell
VL - 174
IS - 1
PB - CELL PRESS
SN - 0092-8674
AB - Neisseria meningitidis, a bacterium responsible for meningitis and septicemia, proliferates and eventually fills the lumen of blood capillaries with multicellular aggregates. The impact of this aggregation process and its specific properties are unknown. We first show that aggregative properties are necessary for efficient infection and study their underlying physical mechanisms. Micropipette aspiration and single-cell tracking unravel unique features of an atypical fluidized phase, with single-cell diffusion exceeding that of isolated cells. A quantitative description of the bacterial pair interactions combined with active matter physics-based modeling show that this behavior relies on type IV pili active dynamics that mediate alternating phases of bacteria fast mutual approach, contact, and release. These peculiar fluid properties proved necessary to adjust to the geometry of capillaries upon bacterial proliferation. Intermittent attractive forces thus generate a fluidized phase that allows for efficient colonization of the blood capillary network during infection.
DO - 10.1016/j.cell.2018.04.010
UR - http://www.dx.doi.org/10.1016/j.cell.2018.04.010
ER -