article · 24/05/2018
Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part II. The Case of Hypericin-Loaded Low-Density Lipoprotein Particles
View at the publisherArticle as PDF
Abstract
The phosphorescence kinetics of singlet oxygen produced by photosensitized hypericin (Hyp) molecules inside low-density lipoprotein (LDL) particles was studied experimentally and by means of numerical and analytical modeling. The phosphorescence signal was measured after short laser pulse irradiation of aqueous Hyp/LDL solutions. The Hyp triplet state lifetime determined by a laser flash-photolysis measurement was 5.3 X 10(-6) s. The numerical and the analytical model described in part I of the present work (DOI: 10.1021/acs.jpcb.8b00658) were used to analyze the observed phosphorescence kinetics of singlet oxygen. It was shown that singlet oxygen diffuses out of LDL particles on a time scale shorter than 0.1 mu s. The total (integrated) concentration of singlet oxygen inside LDL is more than an order of magnitude smaller than the total singlet oxygen concentration in the solvent. The time course of singlet oxygen concentrations inside and outside the particles was calculated using simplified representations of the LDL internal structure. The experimental phosphorescence data were fitted by a linear combination of these concentrations using the emission factor E (the ratio of the radiative singlet oxygen depopulation rate constants inside and outside LDL) as a fitting parameter. The emission factor was determined to be E = 6.7 +/- 2.5. Control measurements were carried out by adding sodium azide, a strong singlet oxygen quencher, to the solution.
Cite this article
Datta, S., Hovan, A., Jutkova, A., Kruglik, S.-G., Jancura, D., Miskovsky, P., & Bano, G. (2018). Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part II. The Case of Hypericin-Loaded Low-Density Lipoprotein Particles. J. Phys. Chem. B, 122(20), 5154-5160.. https://doi.org/10.1021/acs.jpcb.8b00659@article{Datta2018_273,
author = {Datta, Shubhashis and Hovan, Andrej and Jutkova, Annamaria and Kruglik, Sergei G. and Jancura, Daniel and Miskovsky, Pavol and Bano, Gregor},
year = {2018},
month = {5},
title = {Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part II. The Case of Hypericin-Loaded Low-Density Lipoprotein Particles},
journal = {J. Phys. Chem. B},
publisher = {AMER CHEMICAL SOC},
volume = {122},
number = {20},
pages = {5154-5160.},
address = {1155 16TH ST, NW, WASHINGTON, DC 20036 USA},
abstract = {The phosphorescence kinetics of singlet oxygen produced by photosensitized hypericin (Hyp) molecules inside low-density lipoprotein (LDL) particles was studied experimentally and by means of numerical and analytical modeling. The phosphorescence signal was measured after short laser pulse irradiation of aqueous Hyp/LDL solutions. The Hyp triplet state lifetime determined by a laser flash-photolysis measurement was 5.3 X 10(-6) s. The numerical and the analytical model described in part I of the present work (DOI: 10.1021/acs.jpcb.8b00658) were used to analyze the observed phosphorescence kinetics of singlet oxygen. It was shown that singlet oxygen diffuses out of LDL particles on a time scale shorter than 0.1 mu s. The total (integrated) concentration of singlet oxygen inside LDL is more than an order of magnitude smaller than the total singlet oxygen concentration in the solvent. The time course of singlet oxygen concentrations inside and outside the particles was calculated using simplified representations of the LDL internal structure. The experimental phosphorescence data were fitted by a linear combination of these concentrations using the emission factor E (the ratio of the radiative singlet oxygen depopulation rate constants inside and outside LDL) as a fitting parameter. The emission factor was determined to be E = 6.7 +/- 2.5. Control measurements were carried out by adding sodium azide, a strong singlet oxygen quencher, to the solution.},
url = {http://www.dx.doi.org/10.1021/acs.jpcb.8b00659},
doi = {10.1021/acs.jpcb.8b00659},
issn = {1520-6106},
}TY - JOUR
AU - Datta, Shubhashis
AU - Hovan, Andrej
AU - Jutkova, Annamaria
AU - Kruglik, Sergei G.
AU - Jancura, Daniel
AU - Miskovsky, Pavol
AU - Bano, Gregor
PY - 2018
DA - 2018/05/24
TI - Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part II. The Case of Hypericin-Loaded Low-Density Lipoprotein Particles
JO - J. Phys. Chem. B
VL - 122
IS - 20
PB - AMER CHEMICAL SOC
SN - 1520-6106
AB - The phosphorescence kinetics of singlet oxygen produced by photosensitized hypericin (Hyp) molecules inside low-density lipoprotein (LDL) particles was studied experimentally and by means of numerical and analytical modeling. The phosphorescence signal was measured after short laser pulse irradiation of aqueous Hyp/LDL solutions. The Hyp triplet state lifetime determined by a laser flash-photolysis measurement was 5.3 X 10(-6) s. The numerical and the analytical model described in part I of the present work (DOI: 10.1021/acs.jpcb.8b00658) were used to analyze the observed phosphorescence kinetics of singlet oxygen. It was shown that singlet oxygen diffuses out of LDL particles on a time scale shorter than 0.1 mu s. The total (integrated) concentration of singlet oxygen inside LDL is more than an order of magnitude smaller than the total singlet oxygen concentration in the solvent. The time course of singlet oxygen concentrations inside and outside the particles was calculated using simplified representations of the LDL internal structure. The experimental phosphorescence data were fitted by a linear combination of these concentrations using the emission factor E (the ratio of the radiative singlet oxygen depopulation rate constants inside and outside LDL) as a fitting parameter. The emission factor was determined to be E = 6.7 +/- 2.5. Control measurements were carried out by adding sodium azide, a strong singlet oxygen quencher, to the solution.
SP - 5154
EP - 5160.
DO - 10.1021/acs.jpcb.8b00659
UR - http://www.dx.doi.org/10.1021/acs.jpcb.8b00659
ER -