article · 15/06/2012
Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium
Résumé
The directional migration of many cell populations occurs as a coherent group. An amenable model is provided by the posterior lateral line in zebrafish, which is formed by a cohesive primordium that migrates from head to tail and deposits future neuromasts at intervals. We found that prior to the onset of migration, the compact state of the primordium is not fully established, as isolated cells with lateral line identity are present caudal to the main primordium. These isolated cells are retained in position such that they fuse with the migrating primordium as it advances, and later contribute to the leading zone and terminal neuromasts. We found that the isolated lateral line cells are positioned by two antagonistic cues: Fgf signalling attracts them towards the primordium, which counteracts Sdf1 alpha/Cxcr4b-mediated caudal attraction. These findings reveal a novel chemotactic role for Fgf signalling in which it enables the coalescence of the lateral line primordium from an initial fuzzy pattern into a compact group of migrating cells.
Citer cet article
Breau, M.-A., Wilson, D., Wilkinson, D.-G., & Xu, Q. (2012). Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium. Development, 139(12). https://doi.org/10.1242/dev.080275@article{Breau2012_261,
author = {Breau, Marie A. and Wilson, Duncan and Wilkinson, David G. and Xu, Qiling},
year = {2012},
month = {6},
title = {Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium},
journal = {Development},
publisher = {COMPANY OF BIOLOGISTS LTD},
volume = {139},
number = {12},
address = {BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND},
abstract = {The directional migration of many cell populations occurs as a coherent group. An amenable model is provided by the posterior lateral line in zebrafish, which is formed by a cohesive primordium that migrates from head to tail and deposits future neuromasts at intervals. We found that prior to the onset of migration, the compact state of the primordium is not fully established, as isolated cells with lateral line identity are present caudal to the main primordium. These isolated cells are retained in position such that they fuse with the migrating primordium as it advances, and later contribute to the leading zone and terminal neuromasts. We found that the isolated lateral line cells are positioned by two antagonistic cues: Fgf signalling attracts them towards the primordium, which counteracts Sdf1 alpha/Cxcr4b-mediated caudal attraction. These findings reveal a novel chemotactic role for Fgf signalling in which it enables the coalescence of the lateral line primordium from an initial fuzzy pattern into a compact group of migrating cells.},
url = {http://www.dx.doi.org/10.1242/dev.080275},
doi = {10.1242/dev.080275},
issn = {0950-1991},
}TY - JOUR
AU - Breau, Marie A.
AU - Wilson, Duncan
AU - Wilkinson, David G.
AU - Xu, Qiling
PY - 2012
DA - 2012/06/15
TI - Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium
JO - Development
VL - 139
IS - 12
PB - COMPANY OF BIOLOGISTS LTD
SN - 0950-1991
AB - The directional migration of many cell populations occurs as a coherent group. An amenable model is provided by the posterior lateral line in zebrafish, which is formed by a cohesive primordium that migrates from head to tail and deposits future neuromasts at intervals. We found that prior to the onset of migration, the compact state of the primordium is not fully established, as isolated cells with lateral line identity are present caudal to the main primordium. These isolated cells are retained in position such that they fuse with the migrating primordium as it advances, and later contribute to the leading zone and terminal neuromasts. We found that the isolated lateral line cells are positioned by two antagonistic cues: Fgf signalling attracts them towards the primordium, which counteracts Sdf1 alpha/Cxcr4b-mediated caudal attraction. These findings reveal a novel chemotactic role for Fgf signalling in which it enables the coalescence of the lateral line primordium from an initial fuzzy pattern into a compact group of migrating cells.
DO - 10.1242/dev.080275
UR - http://www.dx.doi.org/10.1242/dev.080275
ER -