article · 03/06/2008
A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme
Résumé
The neural crest is generally believed to be the embryonic source of skeletogenic mesenchyme (ectomesenchyme) in the vertebrate head and other derivatives, including pigment cells and neurons and glia of the peripheral nervous system. Although classical transplantation experiments leading to this conclusion assumed that embryonic neural folds were homogeneous epithelia, we reported that embryonic cranial neural folds contain spatially and phenotypically distinct domains, including a lateral nonneural domain with cells that coexpress E-cadherin and PDGFR alpha and a thickened mediodorsal neuroepithelial domain where these proteins are reduced or absent. We now show that Wnt1-Cre is expressed in the lateral nonneural epithelium of rostral neural folds and that cells coexpressing Cre-recombinase and PDGFR alpha delaminate precociously from some of this nonneural epithelium. We also show that ectomesenchymal cells exhibit beta-galactosidase activity in embryos heterozygous for an Ecad-IacZ reporter knockin allele. We conclude that a lateral nonneural domain of the neural fold epithelium, which we call ``metablast,{''} is a source of ectomesenchyme distinct from the neural crest. We suggest that closer analysis of the origin,of ectomesenchyme might help to understand (i) the molecular-genetic regulation of development of both neural crest and ectomesenchyme lineages; (ii) the early developmental origin of skeletogenic and connective tissue mesenchyme in the vertebrate head; and (iii) the presumed origin of head and branchial arch skeletal and connective tissue structures during vertebrate evolution.
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Breau, M.-A., Pietri, T., Stemmler, M.-P., Thiery, J.-P., & Weston, J.-A. (2008). A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme. Proc. Natl. Acad. Sci. U. S. A., 105(22). https://doi.org/10.1073/pnas.0711344105@article{Breau2008_259,
author = {Breau, Marie Anne and Pietri, Thomas and Stemmler, Marc P. and Thiery, Jean Paul and Weston, James A.},
year = {2008},
month = {6},
title = {A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme},
journal = {Proc. Natl. Acad. Sci. U. S. A.},
publisher = {NATL ACAD SCIENCES},
volume = {105},
number = {22},
address = {2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA},
abstract = {The neural crest is generally believed to be the embryonic source of skeletogenic mesenchyme (ectomesenchyme) in the vertebrate head and other derivatives, including pigment cells and neurons and glia of the peripheral nervous system. Although classical transplantation experiments leading to this conclusion assumed that embryonic neural folds were homogeneous epithelia, we reported that embryonic cranial neural folds contain spatially and phenotypically distinct domains, including a lateral nonneural domain with cells that coexpress E-cadherin and PDGFR alpha and a thickened mediodorsal neuroepithelial domain where these proteins are reduced or absent. We now show that Wnt1-Cre is expressed in the lateral nonneural epithelium of rostral neural folds and that cells coexpressing Cre-recombinase and PDGFR alpha delaminate precociously from some of this nonneural epithelium. We also show that ectomesenchymal cells exhibit beta-galactosidase activity in embryos heterozygous for an Ecad-IacZ reporter knockin allele. We conclude that a lateral nonneural domain of the neural fold epithelium, which we call ``metablast,\{''\} is a source of ectomesenchyme distinct from the neural crest. We suggest that closer analysis of the origin,of ectomesenchyme might help to understand (i) the molecular-genetic regulation of development of both neural crest and ectomesenchyme lineages; (ii) the early developmental origin of skeletogenic and connective tissue mesenchyme in the vertebrate head; and (iii) the presumed origin of head and branchial arch skeletal and connective tissue structures during vertebrate evolution.},
url = {http://www.dx.doi.org/10.1073/pnas.0711344105},
doi = {10.1073/pnas.0711344105},
issn = {0027-8424},
}TY - JOUR
AU - Breau, Marie Anne
AU - Pietri, Thomas
AU - Stemmler, Marc P.
AU - Thiery, Jean Paul
AU - Weston, James A.
PY - 2008
DA - 2008/06/03
TI - A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme
JO - Proc. Natl. Acad. Sci. U. S. A.
VL - 105
IS - 22
PB - NATL ACAD SCIENCES
SN - 0027-8424
AB - The neural crest is generally believed to be the embryonic source of skeletogenic mesenchyme (ectomesenchyme) in the vertebrate head and other derivatives, including pigment cells and neurons and glia of the peripheral nervous system. Although classical transplantation experiments leading to this conclusion assumed that embryonic neural folds were homogeneous epithelia, we reported that embryonic cranial neural folds contain spatially and phenotypically distinct domains, including a lateral nonneural domain with cells that coexpress E-cadherin and PDGFR alpha and a thickened mediodorsal neuroepithelial domain where these proteins are reduced or absent. We now show that Wnt1-Cre is expressed in the lateral nonneural epithelium of rostral neural folds and that cells coexpressing Cre-recombinase and PDGFR alpha delaminate precociously from some of this nonneural epithelium. We also show that ectomesenchymal cells exhibit beta-galactosidase activity in embryos heterozygous for an Ecad-IacZ reporter knockin allele. We conclude that a lateral nonneural domain of the neural fold epithelium, which we call ``metablast,{''} is a source of ectomesenchyme distinct from the neural crest. We suggest that closer analysis of the origin,of ectomesenchyme might help to understand (i) the molecular-genetic regulation of development of both neural crest and ectomesenchyme lineages; (ii) the early developmental origin of skeletogenic and connective tissue mesenchyme in the vertebrate head; and (iii) the presumed origin of head and branchial arch skeletal and connective tissue structures during vertebrate evolution.
DO - 10.1073/pnas.0711344105
UR - http://www.dx.doi.org/10.1073/pnas.0711344105
ER -