article · 01/08/2013
A soft cortex is essential for asymmetric spindle positioning in mouse oocytes
Résumé
At mitosis onset, cortical tension increases and cells round up, ensuring correct spindle morphogenesis and orientation. Thus, cortical tension sets up the geometric requirements of cell division. On the contrary, cortical tension decreases during meiotic divisions in mouse oocytes, a puzzling observation because oocytes are round cells, stable in shape, that actively position their spindles. We investigated the pathway leading to reduction in cortical tension and its significance for spindle positioning. We document a previously uncharacterized Arp2/3-dependent thickening of the cortical F-actin essential for first meiotic spindle migration to the cortex. Using micropipette aspiration, we show that cortical tension decreases during meiosis I, resulting from myosin-II exclusion from the cortex, and that cortical F-actin thickening promotes cortical plasticity. These events soften and relax the cortex. They are triggered by the Mos-MAPK pathway and coordinated temporally. Artificial cortex stiffening and theoretical modelling demonstrate that a soft cortex is essential for meiotic spindle positioning.
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Chaigne, A., Campillo, C., Gov, N.-S., Voituriez, R., Azoury, J., Umana-Diaz, C., Almonacid, M., Queguiner, I., Nassoy, P., Sykes, C., Verlhac, M.-H., & Terret, M.-E. (2013). A soft cortex is essential for asymmetric spindle positioning in mouse oocytes. NATURE CELL BIOLOGY, 15(8). https://doi.org/10.1038/ncb2799@article{Chaigne2013_232,
author = {Chaigne, Agathe and Campillo, Clement and Gov, Nir S. and Voituriez, Raphael and Azoury, Jessica and Umana-Diaz, Claudia and Almonacid, Maria and Queguiner, Isabelle and Nassoy, Pierre and Sykes, Cecile and Verlhac, Marie-Helene and Terret, Marie-Emilie},
year = {2013},
month = {8},
title = {A soft cortex is essential for asymmetric spindle positioning in mouse oocytes},
journal = {NATURE CELL BIOLOGY},
volume = {15},
number = {8},
abstract = {At mitosis onset, cortical tension increases and cells round up, ensuring correct spindle morphogenesis and orientation. Thus, cortical tension sets up the geometric requirements of cell division. On the contrary, cortical tension decreases during meiotic divisions in mouse oocytes, a puzzling observation because oocytes are round cells, stable in shape, that actively position their spindles. We investigated the pathway leading to reduction in cortical tension and its significance for spindle positioning. We document a previously uncharacterized Arp2/3-dependent thickening of the cortical F-actin essential for first meiotic spindle migration to the cortex. Using micropipette aspiration, we show that cortical tension decreases during meiosis I, resulting from myosin-II exclusion from the cortex, and that cortical F-actin thickening promotes cortical plasticity. These events soften and relax the cortex. They are triggered by the Mos-MAPK pathway and coordinated temporally. Artificial cortex stiffening and theoretical modelling demonstrate that a soft cortex is essential for meiotic spindle positioning.},
url = {http://www.dx.doi.org/10.1038/ncb2799},
doi = {10.1038/ncb2799},
issn = {1465-7392},
}TY - JOUR
AU - Chaigne, Agathe
AU - Campillo, Clement
AU - Gov, Nir S.
AU - Voituriez, Raphael
AU - Azoury, Jessica
AU - Umana-Diaz, Claudia
AU - Almonacid, Maria
AU - Queguiner, Isabelle
AU - Nassoy, Pierre
AU - Sykes, Cecile
AU - Verlhac, Marie-Helene
AU - Terret, Marie-Emilie
PY - 2013
DA - 2013/08/01
TI - A soft cortex is essential for asymmetric spindle positioning in mouse oocytes
JO - NATURE CELL BIOLOGY
VL - 15
IS - 8
SN - 1465-7392
AB - At mitosis onset, cortical tension increases and cells round up, ensuring correct spindle morphogenesis and orientation. Thus, cortical tension sets up the geometric requirements of cell division. On the contrary, cortical tension decreases during meiotic divisions in mouse oocytes, a puzzling observation because oocytes are round cells, stable in shape, that actively position their spindles. We investigated the pathway leading to reduction in cortical tension and its significance for spindle positioning. We document a previously uncharacterized Arp2/3-dependent thickening of the cortical F-actin essential for first meiotic spindle migration to the cortex. Using micropipette aspiration, we show that cortical tension decreases during meiosis I, resulting from myosin-II exclusion from the cortex, and that cortical F-actin thickening promotes cortical plasticity. These events soften and relax the cortex. They are triggered by the Mos-MAPK pathway and coordinated temporally. Artificial cortex stiffening and theoretical modelling demonstrate that a soft cortex is essential for meiotic spindle positioning.
DO - 10.1038/ncb2799
UR - http://www.dx.doi.org/10.1038/ncb2799
ER -