article · 07/10/2014
Cells as Active Particles in Asymmetric Potentials: Motility under External Gradients
Résumé
Cell migration is a crucial event during development and in disease. Mechanical constraints and chemical gradients can contribute to the establishment of cell direction, but their respective roles remain poorly understood. Using a microfabricated topographical ratchet, we show that the nucleus dictates the direction of cell movement through mechanical guidance by its environment. We demonstrate that this direction can be tuned by combining the topographical ratchet with a biochemical gradient of fibronectin adhesion. We report competition and cooperation between the two external cues. We also quantitatively compare the measurements associated with the trajectory of a model that treats cells as fluctuating particles trapped in a periodic asymmetric potential. We show that the cell nucleus contributes to the strength of the trap, whereas cell protrusions guided by the adhesive gradients add a constant tunable bias to the direction of cell motion.
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Comelles, J., Caballero, D., Voituriez, R., Hortigueela, V., Wollrab, V., Godeau, A.-L., Samitier, J., Martinez, E., & Riveline, D. (2014). Cells as Active Particles in Asymmetric Potentials: Motility under External Gradients. BIOPHYSICAL JOURNAL, 107(7). https://doi.org/10.1016/j.bpj.2014.08.001@article{Comelles2014_214,
author = {Comelles, Jordi and Caballero, David and Voituriez, Raphael and Hortigueela, Veronica and Wollrab, Viktoria and Godeau, Amelie Luise and Samitier, Josep and Martinez, Elena and Riveline, Daniel},
year = {2014},
month = {10},
title = {Cells as Active Particles in Asymmetric Potentials: Motility under External Gradients},
journal = {BIOPHYSICAL JOURNAL},
volume = {107},
number = {7},
abstract = {Cell migration is a crucial event during development and in disease. Mechanical constraints and chemical gradients can contribute to the establishment of cell direction, but their respective roles remain poorly understood. Using a microfabricated topographical ratchet, we show that the nucleus dictates the direction of cell movement through mechanical guidance by its environment. We demonstrate that this direction can be tuned by combining the topographical ratchet with a biochemical gradient of fibronectin adhesion. We report competition and cooperation between the two external cues. We also quantitatively compare the measurements associated with the trajectory of a model that treats cells as fluctuating particles trapped in a periodic asymmetric potential. We show that the cell nucleus contributes to the strength of the trap, whereas cell protrusions guided by the adhesive gradients add a constant tunable bias to the direction of cell motion.},
url = {http://www.dx.doi.org/10.1016/j.bpj.2014.08.001},
doi = {10.1016/j.bpj.2014.08.001},
issn = {0006-3495},
}TY - JOUR
AU - Comelles, Jordi
AU - Caballero, David
AU - Voituriez, Raphael
AU - Hortigueela, Veronica
AU - Wollrab, Viktoria
AU - Godeau, Amelie Luise
AU - Samitier, Josep
AU - Martinez, Elena
AU - Riveline, Daniel
PY - 2014
DA - 2014/10/07
TI - Cells as Active Particles in Asymmetric Potentials: Motility under External Gradients
JO - BIOPHYSICAL JOURNAL
VL - 107
IS - 7
SN - 0006-3495
AB - Cell migration is a crucial event during development and in disease. Mechanical constraints and chemical gradients can contribute to the establishment of cell direction, but their respective roles remain poorly understood. Using a microfabricated topographical ratchet, we show that the nucleus dictates the direction of cell movement through mechanical guidance by its environment. We demonstrate that this direction can be tuned by combining the topographical ratchet with a biochemical gradient of fibronectin adhesion. We report competition and cooperation between the two external cues. We also quantitatively compare the measurements associated with the trajectory of a model that treats cells as fluctuating particles trapped in a periodic asymmetric potential. We show that the cell nucleus contributes to the strength of the trap, whereas cell protrusions guided by the adhesive gradients add a constant tunable bias to the direction of cell motion.
DO - 10.1016/j.bpj.2014.08.001
UR - http://www.dx.doi.org/10.1016/j.bpj.2014.08.001
ER -