article · 01/11/2014
Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes
Résumé
Podosomes are adhesion structures formed in monocyte-derived cells. They are F-actin-rich columns perpendicular to the substrate surrounded by a ring of integrins. Here, to measure podosome protrusive forces, we designed an innovative experimental setup named protrusion force microscopy (PFM), which consists in measuring by atomic force microscopy the deformation induced by living cells onto a compliant Formvar sheet. By quantifying the heights of protrusions made by podosomes onto Formvar sheets, we estimate that a single podosome generates a protrusion force that increases with the stiffness of the substratum, which is a hallmark of mechanosensing activity. We show that the protrusive force generated at podosomes oscillates with a constant period and requires combined actomyosin contraction and actin polymerization. Finally, we elaborate a model to explain the mechanical and oscillatory activities of podosomes. Thus, PFM shows that podosomes are mechanosensing cell structures exerting a protrusive force.
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Labernadie, A., Bouissou, A., Delobelle, P., Balor, S., Voituriez, R., Proag, A., Fourquaux, I., Thibault, C., Vieu, C., Poincloux, R., Charriere, G.-M., & Maridonneau-Parini, I. (2014). Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes. NATURE COMMUNICATIONS, 5(5343). https://doi.org/10.1038/ncomms6343@article{Labernadie2014_213,
author = {Labernadie, Anna and Bouissou, Anais and Delobelle, Patrick and Balor, Stephanie and Voituriez, Raphael and Proag, Amsha and Fourquaux, Isabelle and Thibault, Christophe and Vieu, Christophe and Poincloux, Renaud and Charriere, Guillaume M. and Maridonneau-Parini, Isabelle},
year = {2014},
month = {11},
title = {Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes},
journal = {NATURE COMMUNICATIONS},
volume = {5},
number = {5343},
abstract = {Podosomes are adhesion structures formed in monocyte-derived cells. They are F-actin-rich columns perpendicular to the substrate surrounded by a ring of integrins. Here, to measure podosome protrusive forces, we designed an innovative experimental setup named protrusion force microscopy (PFM), which consists in measuring by atomic force microscopy the deformation induced by living cells onto a compliant Formvar sheet. By quantifying the heights of protrusions made by podosomes onto Formvar sheets, we estimate that a single podosome generates a protrusion force that increases with the stiffness of the substratum, which is a hallmark of mechanosensing activity. We show that the protrusive force generated at podosomes oscillates with a constant period and requires combined actomyosin contraction and actin polymerization. Finally, we elaborate a model to explain the mechanical and oscillatory activities of podosomes. Thus, PFM shows that podosomes are mechanosensing cell structures exerting a protrusive force.},
url = {http://www.dx.doi.org/10.1038/ncomms6343},
doi = {10.1038/ncomms6343},
issn = {2041-1723},
}TY - JOUR
AU - Labernadie, Anna
AU - Bouissou, Anais
AU - Delobelle, Patrick
AU - Balor, Stephanie
AU - Voituriez, Raphael
AU - Proag, Amsha
AU - Fourquaux, Isabelle
AU - Thibault, Christophe
AU - Vieu, Christophe
AU - Poincloux, Renaud
AU - Charriere, Guillaume M.
AU - Maridonneau-Parini, Isabelle
PY - 2014
DA - 2014/11/01
TI - Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes
JO - NATURE COMMUNICATIONS
VL - 5
IS - 5343
SN - 2041-1723
AB - Podosomes are adhesion structures formed in monocyte-derived cells. They are F-actin-rich columns perpendicular to the substrate surrounded by a ring of integrins. Here, to measure podosome protrusive forces, we designed an innovative experimental setup named protrusion force microscopy (PFM), which consists in measuring by atomic force microscopy the deformation induced by living cells onto a compliant Formvar sheet. By quantifying the heights of protrusions made by podosomes onto Formvar sheets, we estimate that a single podosome generates a protrusion force that increases with the stiffness of the substratum, which is a hallmark of mechanosensing activity. We show that the protrusive force generated at podosomes oscillates with a constant period and requires combined actomyosin contraction and actin polymerization. Finally, we elaborate a model to explain the mechanical and oscillatory activities of podosomes. Thus, PFM shows that podosomes are mechanosensing cell structures exerting a protrusive force.
DO - 10.1038/ncomms6343
UR - http://www.dx.doi.org/10.1038/ncomms6343
ER -