article · 21/09/2016
From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide
Résumé
Octreotide and pasireotide are two cyclic somatostatin analogues with an important clinical use in the treatment and diagnosis of neuroendocrine tumors. Herein, by the combined use of several techniques (UV-visible absorption, fluorescence, circular dichroism, zeta-potential, transmission electron microscopy, Raman scattering, surface-enhanced Raman scattering, and quantum mechanical calculations) we have followed the structural dynamics of these analogues in the bulk, as well as their binding sites on plasmonic (gold and silver) colloids. In contrast to the previously derived conclusions, the two peptides seem to possess completely different conformational features. Octreotide, a cyclic octapeptide, is formed by a moderately flexible type-II' beta-turn maintained by a deformable disulfide linkage. Pasireotide, in which the cyclic character is made possible by peptide bonds, manifests a rigid backbone formed by two oppositely placed tight turns of different types, i.e. gamma-turn and type-I beta-turn. Owing to their cationic character, both analogues induce aggregation of negatively charged gold and silver colloids. Nevertheless, despite their notable structural differences, both peptides bind onto gold nanoparticles through their unique (D)-Trp residue. In contrast, their binding to silver colloids seems to be of electrostatic nature, as formed through monodentate or bidentate ionic pairs.
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Hernandez, B., Lopez-Tobar, E., Sanchez-Cortes, S., Coic, Y.-M., Baron, B., Chenal, A., Kruglik, S.-G., Pfluger, F., Cohen, R., & Ghomi, M. (2016). From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide. Physical Chemistry Chemical Physics, 18(35), 24437-24450. https://doi.org/10.1039/c6cp04421b@article{Hernandez2016_21,
author = {Hernandez, Belen and Lopez-Tobar, Eduardo and Sanchez-Cortes, Santiago and Coic, Yves-Marie and Baron, Bruno and Chenal, Alexandre and Kruglik, Sergei G. and Pfluger, Fernando and Cohen, Regis and Ghomi, Mahmoud},
year = {2016},
month = {9},
title = {From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide},
journal = {Physical Chemistry Chemical Physics},
publisher = {ROYAL SOC CHEMISTRY},
volume = {18},
number = {35},
pages = {24437-24450},
address = {THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND},
abstract = {Octreotide and pasireotide are two cyclic somatostatin analogues with an important clinical use in the treatment and diagnosis of neuroendocrine tumors. Herein, by the combined use of several techniques (UV-visible absorption, fluorescence, circular dichroism, zeta-potential, transmission electron microscopy, Raman scattering, surface-enhanced Raman scattering, and quantum mechanical calculations) we have followed the structural dynamics of these analogues in the bulk, as well as their binding sites on plasmonic (gold and silver) colloids. In contrast to the previously derived conclusions, the two peptides seem to possess completely different conformational features. Octreotide, a cyclic octapeptide, is formed by a moderately flexible type-II' beta-turn maintained by a deformable disulfide linkage. Pasireotide, in which the cyclic character is made possible by peptide bonds, manifests a rigid backbone formed by two oppositely placed tight turns of different types, i.e. gamma-turn and type-I beta-turn. Owing to their cationic character, both analogues induce aggregation of negatively charged gold and silver colloids. Nevertheless, despite their notable structural differences, both peptides bind onto gold nanoparticles through their unique (D)-Trp residue. In contrast, their binding to silver colloids seems to be of electrostatic nature, as formed through monodentate or bidentate ionic pairs.},
url = {http://www.dx.doi.org/10.1039/c6cp04421b},
doi = {10.1039/c6cp04421b},
issn = {1463-9076},
}TY - JOUR
AU - Hernandez, Belen
AU - Lopez-Tobar, Eduardo
AU - Sanchez-Cortes, Santiago
AU - Coic, Yves-Marie
AU - Baron, Bruno
AU - Chenal, Alexandre
AU - Kruglik, Sergei G.
AU - Pfluger, Fernando
AU - Cohen, Regis
AU - Ghomi, Mahmoud
PY - 2016
DA - 2016/09/21
TI - From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide
JO - Physical Chemistry Chemical Physics
VL - 18
IS - 35
PB - ROYAL SOC CHEMISTRY
SN - 1463-9076
AB - Octreotide and pasireotide are two cyclic somatostatin analogues with an important clinical use in the treatment and diagnosis of neuroendocrine tumors. Herein, by the combined use of several techniques (UV-visible absorption, fluorescence, circular dichroism, zeta-potential, transmission electron microscopy, Raman scattering, surface-enhanced Raman scattering, and quantum mechanical calculations) we have followed the structural dynamics of these analogues in the bulk, as well as their binding sites on plasmonic (gold and silver) colloids. In contrast to the previously derived conclusions, the two peptides seem to possess completely different conformational features. Octreotide, a cyclic octapeptide, is formed by a moderately flexible type-II' beta-turn maintained by a deformable disulfide linkage. Pasireotide, in which the cyclic character is made possible by peptide bonds, manifests a rigid backbone formed by two oppositely placed tight turns of different types, i.e. gamma-turn and type-I beta-turn. Owing to their cationic character, both analogues induce aggregation of negatively charged gold and silver colloids. Nevertheless, despite their notable structural differences, both peptides bind onto gold nanoparticles through their unique (D)-Trp residue. In contrast, their binding to silver colloids seems to be of electrostatic nature, as formed through monodentate or bidentate ionic pairs.
SP - 24437
EP - 24450
DO - 10.1039/c6cp04421b
UR - http://www.dx.doi.org/10.1039/c6cp04421b
ER -