article · 12/02/2015
Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility
Résumé
3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.
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Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., & Heisenberg, C.-P. (2015). Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility. CELL, 160(4). https://doi.org/10.1016/j.cell.2015.01.008@article{Ruprecht2015_204,
author = {Ruprecht, Verena and Wieser, Stefan and Callan-Jones, Andrew and Smutny, Michael and Morita, Hitoshi and Sako, Keisuke and Barone, Vanessa and Ritsch-Marte, Monika and Sixt, Michael and Voituriez, Raphael and Heisenberg, Carl-Philipp},
year = {2015},
month = {2},
title = {Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility},
journal = {CELL},
volume = {160},
number = {4},
abstract = {3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.},
url = {http://www.dx.doi.org/10.1016/j.cell.2015.01.008},
doi = {10.1016/j.cell.2015.01.008},
issn = {0092-8674},
}TY - JOUR
AU - Ruprecht, Verena
AU - Wieser, Stefan
AU - Callan-Jones, Andrew
AU - Smutny, Michael
AU - Morita, Hitoshi
AU - Sako, Keisuke
AU - Barone, Vanessa
AU - Ritsch-Marte, Monika
AU - Sixt, Michael
AU - Voituriez, Raphael
AU - Heisenberg, Carl-Philipp
PY - 2015
DA - 2015/02/12
TI - Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility
JO - CELL
VL - 160
IS - 4
SN - 0092-8674
AB - 3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.
DO - 10.1016/j.cell.2015.01.008
UR - http://www.dx.doi.org/10.1016/j.cell.2015.01.008
ER -