article · 01/04/2015
Active diffusion positions the nucleus in mouse oocytes
Résumé
In somatic cells, the position of the cell centroid is dictated by the centrosome. The centrosome is instrumental in nucleus positioning, the two structures being physically connected. Mouse oocytes have no centrosomes, yet harbour centrally located nuclei. We demonstrate how oocytes define their geometric centre in the absence of centrosomes. Using live imaging of oocytes, knockout for the formin 2 actin nucleator, with off-centred nuclei, together with optical trapping and modelling, we discover an unprecedented mode of nucleus positioning. We document how active diffusion of actin-coated vesicles, driven by myosin Vb, generates a pressure gradient and a propulsion force sufficient to move the oocyte nucleus. It promotes fluidization of the cytoplasm, contributing to nucleus directional movement towards the centre. Our results highlight the potential of active diffusion, a prominent source of intracellular transport, able to move large organelles such as nuclei, providing in vivo evidence of its biological function.
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Almonacid, M., Ahmed, W.-W., Bussonnier, M., Mailly, P., Betz, T., Voituriez, R., Gov, N.-S., & Verlhac, M.-H. (2015). Active diffusion positions the nucleus in mouse oocytes. NATURE CELL BIOLOGY, 17(4). https://doi.org/10.1038/ncb3131@article{Almonacid2015_202,
author = {Almonacid, Maria and Ahmed, Wylie W. and Bussonnier, Matthias and Mailly, Philippe and Betz, Timo and Voituriez, Raphael and Gov, Nir S. and Verlhac, Marie-Helene},
year = {2015},
month = {4},
title = {Active diffusion positions the nucleus in mouse oocytes},
journal = {NATURE CELL BIOLOGY},
volume = {17},
number = {4},
abstract = {In somatic cells, the position of the cell centroid is dictated by the centrosome. The centrosome is instrumental in nucleus positioning, the two structures being physically connected. Mouse oocytes have no centrosomes, yet harbour centrally located nuclei. We demonstrate how oocytes define their geometric centre in the absence of centrosomes. Using live imaging of oocytes, knockout for the formin 2 actin nucleator, with off-centred nuclei, together with optical trapping and modelling, we discover an unprecedented mode of nucleus positioning. We document how active diffusion of actin-coated vesicles, driven by myosin Vb, generates a pressure gradient and a propulsion force sufficient to move the oocyte nucleus. It promotes fluidization of the cytoplasm, contributing to nucleus directional movement towards the centre. Our results highlight the potential of active diffusion, a prominent source of intracellular transport, able to move large organelles such as nuclei, providing in vivo evidence of its biological function.},
url = {http://www.dx.doi.org/10.1038/ncb3131},
doi = {10.1038/ncb3131},
issn = {1465-7392},
}TY - JOUR
AU - Almonacid, Maria
AU - Ahmed, Wylie W.
AU - Bussonnier, Matthias
AU - Mailly, Philippe
AU - Betz, Timo
AU - Voituriez, Raphael
AU - Gov, Nir S.
AU - Verlhac, Marie-Helene
PY - 2015
DA - 2015/04/01
TI - Active diffusion positions the nucleus in mouse oocytes
JO - NATURE CELL BIOLOGY
VL - 17
IS - 4
SN - 1465-7392
AB - In somatic cells, the position of the cell centroid is dictated by the centrosome. The centrosome is instrumental in nucleus positioning, the two structures being physically connected. Mouse oocytes have no centrosomes, yet harbour centrally located nuclei. We demonstrate how oocytes define their geometric centre in the absence of centrosomes. Using live imaging of oocytes, knockout for the formin 2 actin nucleator, with off-centred nuclei, together with optical trapping and modelling, we discover an unprecedented mode of nucleus positioning. We document how active diffusion of actin-coated vesicles, driven by myosin Vb, generates a pressure gradient and a propulsion force sufficient to move the oocyte nucleus. It promotes fluidization of the cytoplasm, contributing to nucleus directional movement towards the centre. Our results highlight the potential of active diffusion, a prominent source of intracellular transport, able to move large organelles such as nuclei, providing in vivo evidence of its biological function.
DO - 10.1038/ncb3131
UR - http://www.dx.doi.org/10.1038/ncb3131
ER -