article · 01/04/2015
Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors
Résumé
Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity.
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Proag, A., Bouissou, A., Mangeat, T., Voituriez, R., Delobelle, P., Thibault, C., Vieu, C., Maridonneau-Parini, I., & Poincloux, R. (2015). Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors. ACS NANO, 9(4). https://doi.org/10.1021/nn506745r@article{Proag2015_201,
author = {Proag, Amsha and Bouissou, Anais and Mangeat, Thomas and Voituriez, Raphael and Delobelle, Patrick and Thibault, Christophe and Vieu, Christophe and Maridonneau-Parini, Isabelle and Poincloux, Renaud},
year = {2015},
month = {4},
title = {Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors},
journal = {ACS NANO},
volume = {9},
number = {4},
abstract = {Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity.},
url = {http://www.dx.doi.org/10.1021/nn506745r},
doi = {10.1021/nn506745r},
issn = {1936-0851},
}TY - JOUR
AU - Proag, Amsha
AU - Bouissou, Anais
AU - Mangeat, Thomas
AU - Voituriez, Raphael
AU - Delobelle, Patrick
AU - Thibault, Christophe
AU - Vieu, Christophe
AU - Maridonneau-Parini, Isabelle
AU - Poincloux, Renaud
PY - 2015
DA - 2015/04/01
TI - Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors
JO - ACS NANO
VL - 9
IS - 4
SN - 1936-0851
AB - Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity.
DO - 10.1021/nn506745r
UR - http://www.dx.doi.org/10.1021/nn506745r
ER -