article · 21/07/2015
A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish
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Résumé
Zebrafish larva is a unique model for whole-brain functional imaging and to study sensory-motor integration in the vertebrate brain. To take full advantage of this system, one needs to design sensory environments that can mimic the complex spatiotemporal stimulus patterns experienced by the animal in natural conditions. We report on a novel open-ended microfluidic device that delivers pulses of chemical stimuli to agarose-restrained larvae with near-millisecond switching rate and unprecedented spatial and concentration accuracy and reproducibility. In combination with two-photon calcium imaging and recordings of tail movements, we found that stimuli of opposite hedonic values induced different circuit activity patterns. Moreover, by precisely controlling the duration of the stimulus (50-500 ms), we found that the probability of generating a gustatory-induced behavior is encoded by the number of neurons activated. This device may open new ways to dissect the neural-circuit principles underlying chemosensory perception.
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Candelier, R., Murmu, M.-S., Romano, S.-A., Jouary, A., Debregeas, G., & Sumbre, G. (2015). A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish. Scientific Reports, 5(12196). https://doi.org/10.1038/srep12196@article{Candelier2015_2,
author = {Candelier, Raphael and Murmu, Meena Sriti and Romano, Sebastian Alejo and Jouary, Adrien and Debregeas, Georges and Sumbre, German},
year = {2015},
month = {7},
title = {A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish},
journal = {Scientific Reports},
publisher = {NATURE PUBLISHING GROUP},
volume = {5},
number = {12196},
address = {MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND},
abstract = {Zebrafish larva is a unique model for whole-brain functional imaging and to study sensory-motor integration in the vertebrate brain. To take full advantage of this system, one needs to design sensory environments that can mimic the complex spatiotemporal stimulus patterns experienced by the animal in natural conditions. We report on a novel open-ended microfluidic device that delivers pulses of chemical stimuli to agarose-restrained larvae with near-millisecond switching rate and unprecedented spatial and concentration accuracy and reproducibility. In combination with two-photon calcium imaging and recordings of tail movements, we found that stimuli of opposite hedonic values induced different circuit activity patterns. Moreover, by precisely controlling the duration of the stimulus (50-500 ms), we found that the probability of generating a gustatory-induced behavior is encoded by the number of neurons activated. This device may open new ways to dissect the neural-circuit principles underlying chemosensory perception.},
url = {http://www.dx.doi.org/10.1038/srep12196},
doi = {10.1038/srep12196},
issn = {2045-2322},
}TY - JOUR
AU - Candelier, Raphael
AU - Murmu, Meena Sriti
AU - Romano, Sebastian Alejo
AU - Jouary, Adrien
AU - Debregeas, Georges
AU - Sumbre, German
PY - 2015
DA - 2015/07/21
TI - A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish
JO - Scientific Reports
VL - 5
IS - 12196
PB - NATURE PUBLISHING GROUP
SN - 2045-2322
AB - Zebrafish larva is a unique model for whole-brain functional imaging and to study sensory-motor integration in the vertebrate brain. To take full advantage of this system, one needs to design sensory environments that can mimic the complex spatiotemporal stimulus patterns experienced by the animal in natural conditions. We report on a novel open-ended microfluidic device that delivers pulses of chemical stimuli to agarose-restrained larvae with near-millisecond switching rate and unprecedented spatial and concentration accuracy and reproducibility. In combination with two-photon calcium imaging and recordings of tail movements, we found that stimuli of opposite hedonic values induced different circuit activity patterns. Moreover, by precisely controlling the duration of the stimulus (50-500 ms), we found that the probability of generating a gustatory-induced behavior is encoded by the number of neurons activated. This device may open new ways to dissect the neural-circuit principles underlying chemosensory perception.
DO - 10.1038/srep12196
UR - http://www.dx.doi.org/10.1038/srep12196
ER -