article · 01/01/2016
Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells
Résumé
Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA mDial-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42 Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4 MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDial-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.
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Vargas, P., Maiuri, P., Bretou, M., Saez, P.-J., Pierobon, P., Maurin, M., Chabaud, M., Lankar, D., Obino, D., Terriac, E., Raab, M., Thiam, H.-R., Brocker, T., Kitchen-Goosen, S.-M., Alberts, A.-S., Sunareni, P., Xia, S., Li, R., Voituriez, R., Piel, M., & Lennon-Dumenil, A.-M. (2016). Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells. NATURE CELL BIOLOGY, 18(1). https://doi.org/10.1038/ncb3284@article{Vargas2016_184,
author = {Vargas, Pablo and Maiuri, Paolo and Bretou, Marine and Saez, Pablo J. and Pierobon, Paolo and Maurin, Mathieu and Chabaud, Melanie and Lankar, Danielle and Obino, Dorian and Terriac, Emmanuel and Raab, Matthew and Thiam, Hawa-Racine and Brocker, Thomas and Kitchen-Goosen, Susan M. and Alberts, Arthur S. and Sunareni, Praveen and Xia, Sheng and Li, Rong and Voituriez, Raphael and Piel, Matthieu and Lennon-Dumenil, Ana-Maria},
year = {2016},
month = {1},
title = {Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells},
journal = {NATURE CELL BIOLOGY},
volume = {18},
number = {1},
abstract = {Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA mDial-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42 Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4 MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDial-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.},
url = {http://www.dx.doi.org/10.1038/ncb3284},
doi = {10.1038/ncb3284},
issn = {1465-7392},
}TY - JOUR
AU - Vargas, Pablo
AU - Maiuri, Paolo
AU - Bretou, Marine
AU - Saez, Pablo J.
AU - Pierobon, Paolo
AU - Maurin, Mathieu
AU - Chabaud, Melanie
AU - Lankar, Danielle
AU - Obino, Dorian
AU - Terriac, Emmanuel
AU - Raab, Matthew
AU - Thiam, Hawa-Racine
AU - Brocker, Thomas
AU - Kitchen-Goosen, Susan M.
AU - Alberts, Arthur S.
AU - Sunareni, Praveen
AU - Xia, Sheng
AU - Li, Rong
AU - Voituriez, Raphael
AU - Piel, Matthieu
AU - Lennon-Dumenil, Ana-Maria
PY - 2016
DA - 2016/01/01
TI - Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells
JO - NATURE CELL BIOLOGY
VL - 18
IS - 1
SN - 1465-7392
AB - Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA mDial-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42 Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4 MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDial-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.
DO - 10.1038/ncb3284
UR - http://www.dx.doi.org/10.1038/ncb3284
ER -