article · 11/09/2017
Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle
Résumé
Nuclear movements are important for multiple cellular functions, and are driven by polarized forces generated by motor proteins and the cytoskeleton. During skeletal myofibre formation or regeneration, nuclei move from the centre to the periphery of the myofibre for proper muscle function. Centrally located nuclei are also found in different muscle disorders. Using theoretical and experimental approaches, we demonstrate that nuclear movement to the periphery of myofibres is mediated by centripetal forces around the nucleus. These forces arise from myofibril contraction and crosslinking that `zip' around the nucleus in combination with tight regulation of nuclear stiffness by lamin A/C. In addition, an Arp2/3 complex containing Arpc5L together with gamma-actin is required to organize desmin to crosslink myofibrils for nuclear movement. Our work reveals that centripetal forces exerted by myofibrils squeeze the nucleus to the periphery of myofibres.
Citer cet article
Roman, W., Martins, J.-P., Carvalho, F.-A., Voituriez, R., Abella, J.-V.-G., Santos, N.-C., Cadot, B., Way, M., & Gomes, E.-R. (2017). Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle. NATURE CELL BIOLOGY, 19(10). https://doi.org/10.1038/ncb3605@article{Roman2017_167,
author = {Roman, William and Martins, Joao P. and Carvalho, Filomena A. and Voituriez, Raphael and Abella, Jasmine V. G. and Santos, Nuno C. and Cadot, Bruno and Way, Michael and Gomes, Edgar R.},
year = {2017},
month = {9},
title = {Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle},
journal = {NATURE CELL BIOLOGY},
volume = {19},
number = {10},
abstract = {Nuclear movements are important for multiple cellular functions, and are driven by polarized forces generated by motor proteins and the cytoskeleton. During skeletal myofibre formation or regeneration, nuclei move from the centre to the periphery of the myofibre for proper muscle function. Centrally located nuclei are also found in different muscle disorders. Using theoretical and experimental approaches, we demonstrate that nuclear movement to the periphery of myofibres is mediated by centripetal forces around the nucleus. These forces arise from myofibril contraction and crosslinking that `zip' around the nucleus in combination with tight regulation of nuclear stiffness by lamin A/C. In addition, an Arp2/3 complex containing Arpc5L together with gamma-actin is required to organize desmin to crosslink myofibrils for nuclear movement. Our work reveals that centripetal forces exerted by myofibrils squeeze the nucleus to the periphery of myofibres.},
url = {http://www.dx.doi.org/10.1038/ncb3605},
doi = {10.1038/ncb3605},
issn = {1465-7392},
}TY - JOUR
AU - Roman, William
AU - Martins, Joao P.
AU - Carvalho, Filomena A.
AU - Voituriez, Raphael
AU - Abella, Jasmine V. G.
AU - Santos, Nuno C.
AU - Cadot, Bruno
AU - Way, Michael
AU - Gomes, Edgar R.
PY - 2017
DA - 2017/09/11
TI - Myofibril contraction and crosslinking drive nuclear movement to the periphery of skeletal muscle
JO - NATURE CELL BIOLOGY
VL - 19
IS - 10
SN - 1465-7392
AB - Nuclear movements are important for multiple cellular functions, and are driven by polarized forces generated by motor proteins and the cytoskeleton. During skeletal myofibre formation or regeneration, nuclei move from the centre to the periphery of the myofibre for proper muscle function. Centrally located nuclei are also found in different muscle disorders. Using theoretical and experimental approaches, we demonstrate that nuclear movement to the periphery of myofibres is mediated by centripetal forces around the nucleus. These forces arise from myofibril contraction and crosslinking that `zip' around the nucleus in combination with tight regulation of nuclear stiffness by lamin A/C. In addition, an Arp2/3 complex containing Arpc5L together with gamma-actin is required to organize desmin to crosslink myofibrils for nuclear movement. Our work reveals that centripetal forces exerted by myofibrils squeeze the nucleus to the periphery of myofibres.
DO - 10.1038/ncb3605
UR - http://www.dx.doi.org/10.1038/ncb3605
ER -