article · 15/04/2010
Interaction dynamics of hypericin with low-density lipoproteins and U87-MG cells
Résumé
The natural photosensitizer hypericin exhibits potent properties for tumor diagnosis and photodynamic therapy. Fluorescent properties of hypericin along with various technical approaches have been used for dynamic studies of its interaction with low-density lipoprotein and U87 glioma cells. Evidences for hypericin release from low-density lipoprotein towards cells plasmatic membrane are addressed. Subsequent subcellular bulk flow redistribution leading to non-specific staining of intracellular membranes compartment were observed within cells. It was shown, that monomers of hypericin are the only redistributive forms. Increasing concentration of hypericin leads to the formation of non-fluorescent aggregates within low-density lipoprotein as well as within the U87 cells, and can preclude its photosensitizing activities. However, the aggregation process can only account for a part of the observed emission decrease. As shown by the excited state lifetime measurements, this fluorescence quenching actually results from a combination of aggregation process and energy transfer from monomers to aggregates. In all experiments, hydrophobic character of hypericin appears as the driving force of its redistribution process. (C) 2010 Elsevier B.V. All rights reserved.
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Huntosova, V., Alvarez, L., Bryndzova, L., Nadova, Z., Jancura, D., Buriankova, L., Bonneau, S., Brault, D., Miskovsky, P., & Sureau, F. (2010). Interaction dynamics of hypericin with low-density lipoproteins and U87-MG cells. International Journal of Pharmaceutics, 389(1-2). https://doi.org/10.1016/j.ijpharm.2010.01.010@article{Huntosova2010_150,
author = {Huntosova, Veronika and Alvarez, Luis and Bryndzova, Lenka and Nadova, Zuzana and Jancura, Daniel and Buriankova, Luboslava and Bonneau, Stephanie and Brault, Daniel and Miskovsky, Pavol and Sureau, Franck},
year = {2010},
month = {4},
title = {Interaction dynamics of hypericin with low-density lipoproteins and U87-MG cells},
journal = {International Journal of Pharmaceutics},
volume = {389},
number = {1-2},
abstract = {The natural photosensitizer hypericin exhibits potent properties for tumor diagnosis and photodynamic therapy. Fluorescent properties of hypericin along with various technical approaches have been used for dynamic studies of its interaction with low-density lipoprotein and U87 glioma cells. Evidences for hypericin release from low-density lipoprotein towards cells plasmatic membrane are addressed. Subsequent subcellular bulk flow redistribution leading to non-specific staining of intracellular membranes compartment were observed within cells. It was shown, that monomers of hypericin are the only redistributive forms. Increasing concentration of hypericin leads to the formation of non-fluorescent aggregates within low-density lipoprotein as well as within the U87 cells, and can preclude its photosensitizing activities. However, the aggregation process can only account for a part of the observed emission decrease. As shown by the excited state lifetime measurements, this fluorescence quenching actually results from a combination of aggregation process and energy transfer from monomers to aggregates. In all experiments, hydrophobic character of hypericin appears as the driving force of its redistribution process. (C) 2010 Elsevier B.V. All rights reserved.},
url = {http://www.dx.doi.org/10.1016/j.ijpharm.2010.01.010},
doi = {10.1016/j.ijpharm.2010.01.010},
issn = {0378-5173},
}TY - JOUR
AU - Huntosova, Veronika
AU - Alvarez, Luis
AU - Bryndzova, Lenka
AU - Nadova, Zuzana
AU - Jancura, Daniel
AU - Buriankova, Luboslava
AU - Bonneau, Stephanie
AU - Brault, Daniel
AU - Miskovsky, Pavol
AU - Sureau, Franck
PY - 2010
DA - 2010/04/15
TI - Interaction dynamics of hypericin with low-density lipoproteins and U87-MG cells
JO - International Journal of Pharmaceutics
VL - 389
IS - 1-2
SN - 0378-5173
AB - The natural photosensitizer hypericin exhibits potent properties for tumor diagnosis and photodynamic therapy. Fluorescent properties of hypericin along with various technical approaches have been used for dynamic studies of its interaction with low-density lipoprotein and U87 glioma cells. Evidences for hypericin release from low-density lipoprotein towards cells plasmatic membrane are addressed. Subsequent subcellular bulk flow redistribution leading to non-specific staining of intracellular membranes compartment were observed within cells. It was shown, that monomers of hypericin are the only redistributive forms. Increasing concentration of hypericin leads to the formation of non-fluorescent aggregates within low-density lipoprotein as well as within the U87 cells, and can preclude its photosensitizing activities. However, the aggregation process can only account for a part of the observed emission decrease. As shown by the excited state lifetime measurements, this fluorescence quenching actually results from a combination of aggregation process and energy transfer from monomers to aggregates. In all experiments, hydrophobic character of hypericin appears as the driving force of its redistribution process. (C) 2010 Elsevier B.V. All rights reserved.
DO - 10.1016/j.ijpharm.2010.01.010
UR - http://www.dx.doi.org/10.1016/j.ijpharm.2010.01.010
ER -