article · 01/01/2009
Microfluidic stickers for cell- and tissue-based assays in microchannels
Résumé
Difficulties in culturing cells inside microchannels is a major obstacle for the wide use of microfluidic technology in cell biology. Here, we present a simple and versatile method to interface closed microchannels with cellular and multicellular systems. Our approach, based on microfluidic stickers which can adhere to wet glass coverslips, eliminates the need to adapt cell culture conditions to microchannels and greatly facilitates the methods required to position cells into microcircuits. We demonstrate the simplicity and efficiency of the method with HeLa cells, primary cultured neurons and Drosophila tissues.
Citer cet article
Morel, M., Bartolo, D., Galas, J.-C., Dahan, M., & Studer, V. (2009). Microfluidic stickers for cell- and tissue-based assays in microchannels. Lab Chip, 9(7). https://doi.org/10.1039/b819090a@article{Morel2009_118,
author = {Morel, Mathieu and Bartolo, Denis and Galas, Jean-Christophe and Dahan, Maxime and Studer, Vincent},
year = {2009},
month = {1},
title = {Microfluidic stickers for cell- and tissue-based assays in microchannels},
journal = {Lab Chip},
publisher = {ROYAL SOC CHEMISTRY},
volume = {9},
number = {7},
address = {THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND},
abstract = {Difficulties in culturing cells inside microchannels is a major obstacle for the wide use of microfluidic technology in cell biology. Here, we present a simple and versatile method to interface closed microchannels with cellular and multicellular systems. Our approach, based on microfluidic stickers which can adhere to wet glass coverslips, eliminates the need to adapt cell culture conditions to microchannels and greatly facilitates the methods required to position cells into microcircuits. We demonstrate the simplicity and efficiency of the method with HeLa cells, primary cultured neurons and Drosophila tissues.},
url = {http://www.dx.doi.org/10.1039/b819090a},
doi = {10.1039/b819090a},
issn = {1473-0197},
}TY - JOUR
AU - Morel, Mathieu
AU - Bartolo, Denis
AU - Galas, Jean-Christophe
AU - Dahan, Maxime
AU - Studer, Vincent
PY - 2009
DA - 2009/01/01
TI - Microfluidic stickers for cell- and tissue-based assays in microchannels
JO - Lab Chip
VL - 9
IS - 7
PB - ROYAL SOC CHEMISTRY
SN - 1473-0197
AB - Difficulties in culturing cells inside microchannels is a major obstacle for the wide use of microfluidic technology in cell biology. Here, we present a simple and versatile method to interface closed microchannels with cellular and multicellular systems. Our approach, based on microfluidic stickers which can adhere to wet glass coverslips, eliminates the need to adapt cell culture conditions to microchannels and greatly facilitates the methods required to position cells into microcircuits. We demonstrate the simplicity and efficiency of the method with HeLa cells, primary cultured neurons and Drosophila tissues.
DO - 10.1039/b819090a
UR - http://www.dx.doi.org/10.1039/b819090a
ER -