article · 01/01/2012
Concentration landscape generators for shear free dynamic chemical stimulation
Résumé
In this paper we first introduce a novel fabrication process, which allows for easy integration of thin track-etched nanoporous membranes, within 2D or 3D microchannel networks. In these networks, soluble chemical compounds can diffuse out of the channels through well-defined and spatially organized microfabricated porous openings. Interestingly, multiple micron-scale porous areas can be integrated in the same device and each of these areas can be connected to a different microfluidic channel and reservoir. We then present and characterize several membrane-based microdevices and their use for the generation of stable diffusible concentration gradients and complex dynamic chemical landscapes under shear free conditions. We also demonstrate how a simple flow-focusing geometry can be used to generate ``on-demand'' concentration profiles. In turn, these devices should provide an ideal experimental framework for high throughput cell-based assays: long term high-resolution video microscopy experiments can be performed, under multiple spatially and temporally controlled chemical conditions, with simple protocols and in a cell-friendly environment.
Citer cet article
Morel, M., Galas, J.-C., Dahan, M., & Studer, V. (2012). Concentration landscape generators for shear free dynamic chemical stimulation. Lab Chip, 12(7). https://doi.org/10.1039/c2lc20994b@article{Morel2012_112,
author = {Morel, Mathieu and Galas, Jean-Christophe and Dahan, Maxime and Studer, Vincent},
year = {2012},
month = {1},
title = {Concentration landscape generators for shear free dynamic chemical stimulation},
journal = {Lab Chip},
publisher = {ROYAL SOC CHEMISTRY},
volume = {12},
number = {7},
address = {THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND},
abstract = {In this paper we first introduce a novel fabrication process, which allows for easy integration of thin track-etched nanoporous membranes, within 2D or 3D microchannel networks. In these networks, soluble chemical compounds can diffuse out of the channels through well-defined and spatially organized microfabricated porous openings. Interestingly, multiple micron-scale porous areas can be integrated in the same device and each of these areas can be connected to a different microfluidic channel and reservoir. We then present and characterize several membrane-based microdevices and their use for the generation of stable diffusible concentration gradients and complex dynamic chemical landscapes under shear free conditions. We also demonstrate how a simple flow-focusing geometry can be used to generate ``on-demand'' concentration profiles. In turn, these devices should provide an ideal experimental framework for high throughput cell-based assays: long term high-resolution video microscopy experiments can be performed, under multiple spatially and temporally controlled chemical conditions, with simple protocols and in a cell-friendly environment.},
url = {http://www.dx.doi.org/10.1039/c2lc20994b},
doi = {10.1039/c2lc20994b},
issn = {1473-0197},
}TY - JOUR
AU - Morel, Mathieu
AU - Galas, Jean-Christophe
AU - Dahan, Maxime
AU - Studer, Vincent
PY - 2012
DA - 2012/01/01
TI - Concentration landscape generators for shear free dynamic chemical stimulation
JO - Lab Chip
VL - 12
IS - 7
PB - ROYAL SOC CHEMISTRY
SN - 1473-0197
AB - In this paper we first introduce a novel fabrication process, which allows for easy integration of thin track-etched nanoporous membranes, within 2D or 3D microchannel networks. In these networks, soluble chemical compounds can diffuse out of the channels through well-defined and spatially organized microfabricated porous openings. Interestingly, multiple micron-scale porous areas can be integrated in the same device and each of these areas can be connected to a different microfluidic channel and reservoir. We then present and characterize several membrane-based microdevices and their use for the generation of stable diffusible concentration gradients and complex dynamic chemical landscapes under shear free conditions. We also demonstrate how a simple flow-focusing geometry can be used to generate ``on-demand'' concentration profiles. In turn, these devices should provide an ideal experimental framework for high throughput cell-based assays: long term high-resolution video microscopy experiments can be performed, under multiple spatially and temporally controlled chemical conditions, with simple protocols and in a cell-friendly environment.
DO - 10.1039/c2lc20994b
UR - http://www.dx.doi.org/10.1039/c2lc20994b
ER -