article · 17/10/2012
Amplification and Temporal Filtering during Gradient Sensing by Nerve Growth Cones Probed with a Microfluidic Assay
Résumé
Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. To ensure a sensitive and robust response to directional signals in complex and dynamic chemical landscapes, GCs are presumably able to amplify and filter external information. How these processing tasks are performed remains however poorly known. Here, we probe the signal-processing capabilities of single GCs during gamma-Aminobutyric acid (GABA) directional sensing with a shear-free microfluidic assay that enables systematic measurements of the GC output response to variable input gradients. By measuring at the single molecule level the polarization of GABA(A) chemoreceptors at the GC membrane, as a function of the external GABA gradient, we find that GCs act as i), signal amplifiers over a narrow range of concentrations, and ii), low-pass temporal filters with a cutoff frequency independent of stimuli conditions. With computational modeling, we determine that these systems-level properties arise at a molecular level from the saturable occupancy response and the lateral dynamics of GABA(A) receptors.
Citer cet article
Morel, M., Shynkar, V., Galas, J.-C., Dupin, I., Bouzigues, C., Studer, V., & Dahan, M. (2012). Amplification and Temporal Filtering during Gradient Sensing by Nerve Growth Cones Probed with a Microfluidic Assay. Biophys. J., 103(8). https://doi.org/10.1016/j.bpj.2012.08.040@article{Morel2012_110,
author = {Morel, Mathieu and Shynkar, Vasyl and Galas, Jean-Christophe and Dupin, Isabelle and Bouzigues, Cedric and Studer, Vincent and Dahan, Maxime},
year = {2012},
month = {10},
title = {Amplification and Temporal Filtering during Gradient Sensing by Nerve Growth Cones Probed with a Microfluidic Assay},
journal = {Biophys. J.},
publisher = {CELL PRESS},
volume = {103},
number = {8},
address = {600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA},
abstract = {Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. To ensure a sensitive and robust response to directional signals in complex and dynamic chemical landscapes, GCs are presumably able to amplify and filter external information. How these processing tasks are performed remains however poorly known. Here, we probe the signal-processing capabilities of single GCs during gamma-Aminobutyric acid (GABA) directional sensing with a shear-free microfluidic assay that enables systematic measurements of the GC output response to variable input gradients. By measuring at the single molecule level the polarization of GABA(A) chemoreceptors at the GC membrane, as a function of the external GABA gradient, we find that GCs act as i), signal amplifiers over a narrow range of concentrations, and ii), low-pass temporal filters with a cutoff frequency independent of stimuli conditions. With computational modeling, we determine that these systems-level properties arise at a molecular level from the saturable occupancy response and the lateral dynamics of GABA(A) receptors.},
url = {http://www.dx.doi.org/10.1016/j.bpj.2012.08.040},
doi = {10.1016/j.bpj.2012.08.040},
issn = {0006-3495},
}TY - JOUR
AU - Morel, Mathieu
AU - Shynkar, Vasyl
AU - Galas, Jean-Christophe
AU - Dupin, Isabelle
AU - Bouzigues, Cedric
AU - Studer, Vincent
AU - Dahan, Maxime
PY - 2012
DA - 2012/10/17
TI - Amplification and Temporal Filtering during Gradient Sensing by Nerve Growth Cones Probed with a Microfluidic Assay
JO - Biophys. J.
VL - 103
IS - 8
PB - CELL PRESS
SN - 0006-3495
AB - Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. To ensure a sensitive and robust response to directional signals in complex and dynamic chemical landscapes, GCs are presumably able to amplify and filter external information. How these processing tasks are performed remains however poorly known. Here, we probe the signal-processing capabilities of single GCs during gamma-Aminobutyric acid (GABA) directional sensing with a shear-free microfluidic assay that enables systematic measurements of the GC output response to variable input gradients. By measuring at the single molecule level the polarization of GABA(A) chemoreceptors at the GC membrane, as a function of the external GABA gradient, we find that GCs act as i), signal amplifiers over a narrow range of concentrations, and ii), low-pass temporal filters with a cutoff frequency independent of stimuli conditions. With computational modeling, we determine that these systems-level properties arise at a molecular level from the saturable occupancy response and the lateral dynamics of GABA(A) receptors.
DO - 10.1016/j.bpj.2012.08.040
UR - http://www.dx.doi.org/10.1016/j.bpj.2012.08.040
ER -